Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DSTN antibody [EPR15827(B)] (ab186754)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15827(B)] to DSTN
  • Suitable for: ICC/IF, WB, IHC-P, Flow Cyt, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-DSTN antibody [EPR15827(B)]
    See all DSTN primary antibodies

  • Description

    Rabbit monoclonal [EPR15827(B)] to DSTN

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab128429)

  • Positive control

    • Recombinant Human DSTN protein (ab128429) can be used as a positive control in WB. MCF-7, HeLa, A549, C6, PC-12 and NIH/3T3 cell lysates; Human bladder transitional cell carcinoma tissue; HeLa and MCF-7 cells.

  • General notes

     

     

    Previously labelled as Destrin. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15827(B)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-DSTN antibody [EPR15827(B)] (ab186754)
    Western blot - Anti-DSTN antibody [EPR15827(B)] (ab186754)
    Anti-DSTN antibody [EPR15827(B)] (ab186754) at 1/10000 dilution + MCF-7 cell lyate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 19 kDa
    Observed band size: 17 kDa
    why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-DSTN antibody [EPR15827(B)] (ab186754)
    Immunocytochemistry/ Immunofluorescence - Anti-DSTN antibody [EPR15827(B)] (ab186754)

    ab186754 staining DSTN in MCF-7 (human breast carcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol.. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

    Negative control 1:PBS only.

  • Western blot - Anti-DSTN antibody [EPR15827(B)] (ab186754)
    Western blot - Anti-DSTN antibody [EPR15827(B)] (ab186754)
    All lanes : Anti-DSTN antibody [EPR15827(B)] (ab186754) at 1/2000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 19 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-DSTN antibody [EPR15827(B)] (ab186754)
    Western blot - Anti-DSTN antibody [EPR15827(B)] (ab186754)
    All lanes : Anti-DSTN antibody [EPR15827(B)] (ab186754) at 1/2000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : PC-12 cell lysate
    Lane 3 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 19 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DSTN antibody [EPR15827(B)] (ab186754)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DSTN antibody [EPR15827(B)] (ab186754)

    Immunohistochemical analysis of paraffin-embedded Human bladder transitional cell carcinoma tissue labeling DSTN with ab186754 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-DSTN antibody [EPR15827(B)] (ab186754)
    Immunocytochemistry/ Immunofluorescence - Anti-DSTN antibody [EPR15827(B)] (ab186754)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling DSTN with ab186754 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-DSTN antibody [EPR15827(B)] (ab186754)
    Immunocytochemistry/ Immunofluorescence - Anti-DSTN antibody [EPR15827(B)] (ab186754)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF-7 cells labeling DSTN with ab186754 at 1/1000 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

  • Flow Cytometry - Anti-DSTN antibody [EPR15827(B)] (ab186754)
    Flow Cytometry - Anti-DSTN antibody [EPR15827(B)] (ab186754)

    Flow cytometric analysis of 2% paraformaldehyde-fixed MCF-7 cells labeling DSTN with ab186754 at 1/360 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

  • Immunoprecipitation - Anti-DSTN antibody [EPR15827(B)] (ab186754)
    Immunoprecipitation - Anti-DSTN antibody [EPR15827(B)] (ab186754)

    Western blot analysis of DSTN in A549 cell lysate immunoprecipitated with ab186754 at 1/50 dilution. 

    Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

  • Anti-DSTN antibody [EPR15827(B)] (ab186754)
    Anti-DSTN antibody [EPR15827(B)] (ab186754)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-DSTN antibody [EPR15827(B)] (ab186754)

© 2021 Astana Biomed Group LLP. All rights reserved.