Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸ 10 µl 130 665 ₸

Order now and get it on Tuesday March 09, 2021

Anti-DSTN antibody [EPR15827(B)] (ab186754)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15827(B)] to DSTN
Suitable for: ICC/IF, WB, IHC-P, Flow Cyt, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-DSTN antibody [EPR15827(B)]
See all DSTN primary antibodies
Description

Rabbit monoclonal [EPR15827(B)] to DSTN
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available as ab128429)
Positive control
- Recombinant Human DSTN protein (ab128429) can be used as a positive control in WB. MCF-7, HeLa, A549, C6, PC-12 and NIH/3T3 cell lysates; Human bladder transitional cell carcinoma tissue; HeLa and MCF-7 cells.
General notes
Previously labelled as Destrin.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15827(B)
Isotype

IgG
Research areas

Western blot - Anti-DSTN antibody [EPR15827(B)] (ab186754)

Anti-DSTN antibody [EPR15827(B)] (ab186754) at 1/10000 dilution + MCF-7 cell lyate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 19 kDa
Observed band size: 17 kDa
why is the actual band size different from the predicted?
Immunocytochemistry/ Immunofluorescence - Anti-DSTN antibody [EPR15827(B)] (ab186754)

ab186754 staining DSTN in MCF-7 (human breast carcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol.. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

Negative control 1:PBS only.
Western blot - Anti-DSTN antibody [EPR15827(B)] (ab186754)

All lanes : Anti-DSTN antibody [EPR15827(B)] (ab186754) at 1/2000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 19 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Western blot - Anti-DSTN antibody [EPR15827(B)] (ab186754)

All lanes : Anti-DSTN antibody [EPR15827(B)] (ab186754) at 1/2000 dilution

Lane 1 : C6 cell lysate
Lane 2 : PC-12 cell lysate
Lane 3 : NIH/3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 19 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DSTN antibody [EPR15827(B)] (ab186754)

Immunohistochemical analysis of paraffin-embedded Human bladder transitional cell carcinoma tissue labeling DSTN with ab186754 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-DSTN antibody [EPR15827(B)] (ab186754)

Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling DSTN with ab186754 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).
Immunocytochemistry/ Immunofluorescence - Anti-DSTN antibody [EPR15827(B)] (ab186754)

Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF-7 cells labeling DSTN with ab186754 at 1/1000 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).
Flow Cytometry - Anti-DSTN antibody [EPR15827(B)] (ab186754)

Flow cytometric analysis of 2% paraformaldehyde-fixed MCF-7 cells labeling DSTN with ab186754 at 1/360 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
Immunoprecipitation - Anti-DSTN antibody [EPR15827(B)] (ab186754)

Western blot analysis of DSTN in A549 cell lysate immunoprecipitated with ab186754 at 1/50 dilution.

Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
Anti-DSTN antibody [EPR15827(B)] (ab186754)

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