Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 09, 2021

Anti-DSTN antibody [EPR15828] (ab192262)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15828] to DSTN
Suitable for: WB, IHC-P, ICC/IF, IP
Reacts with: Human

Product name

Anti-DSTN antibody [EPR15828]
See all DSTN primary antibodies
Description

Rabbit monoclonal [EPR15828] to DSTN
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- MCF-7, HepG2, HeLa and A549 cell lysate. Human cervical carcinoma tissue. MCF-7 cells.
General notes
Previously labelled as Destrin.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15828
Isotype

IgG
Research areas

Western blot - Anti-DSTN antibody [EPR15828] (ab192262)

All lanes : Anti-DSTN antibody [EPR15828] (ab192262) at 1/5000 dilution

Lane 1 : MCF-7 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : A549 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 19 kDa
Immunocytochemistry/ Immunofluorescence - Anti-DSTN antibody [EPR15828] (ab192262)

Immunofluorescent analysis of 4% formaldehyde fixed MCF-7 cells labeling DSTN using ab192262 at a 1/50 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) ab150077 was used as the secondary at a 1/200 dilution. Permeabilized using 0.1% Triton X-100. The two negative controls; 1. Primary ab concentration (anti-DSTN) is 1:50 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1:500 dilution; 2. Primary ab concentration (anti-DSTN) is 1:50 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1:500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DSTN antibody [EPR15828] (ab192262)

Immunohistochemical analysis of paraffin embedded human cervical carcinoma tissue sections labeling DSTN using ab192262 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-DSTN antibody [EPR15828] (ab192262)

Lysate from A549 cells was immunoprecipitated with ab192262 at a 1/30 dilution. For the subsequent blot, ab192262 used at a 1/1000 dilution with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a 1/1000 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST.
Anti-DSTN antibody [EPR15828] (ab192262)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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