Anti-Angiogenin antibody (ab95389)
Key features and details
- Rabbit polyclonal to Angiogenin
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Angiogenin antibody
See all Angiogenin primary antibodies -
Description
Rabbit polyclonal to Angiogenin -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rabbit, Chimpanzee
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Immunogen
Synthetic peptide corresponding to Human Angiogenin aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab107903) -
Positive control
- Purchase matching WB positive control:Recombinant Human Angiogenin protein
- Recombinant Human Angiogenin protein (ab114413) can be used as a positive control in WB. This antibody gave a positive signal in the following human tissue lysates: Lung; Liver.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab95389 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 mg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 17 kDa). ICC/IF Use a concentration of 5 µg/ml. Target
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Function
May function as a tRNA-specific ribonuclease that abolishes protein synthesis by specifically hydrolyzing cellular tRNAs. Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo. -
Tissue specificity
Expressed predominantly in the liver. Also detected in endothelial cells and spinal cord neurons. -
Involvement in disease
Defects in ANG are the cause of susceptibility to amyotrophic lateral sclerosis type 9 (ALS9) [MIM:611895]. ALS is a degenerative disorder of motor neurons in the cortex, brain stem and spinal cord. ALS is characterized by muscular weakness and atrophy. -
Sequence similarities
Belongs to the pancreatic ribonuclease family. -
Developmental stage
Low level expression in the developing fetus, increased in the neonate, and maximal in the adult. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 494026 Chimpanzee
- Entrez Gene: 283 Human
- Entrez Gene: 11727 Mouse
- Omim: 105850 Human
- SwissProt: Q8WME8 Chimpanzee
- SwissProt: P03950 Human
- SwissProt: P21570 Mouse
- SwissProt: P31347 Rabbit
see all -
Alternative names
- ALS9 antibody
- AMYOTROPHIC LATERAL SCLEROSIS antibody
- ANG 1 antibody
see all
Images
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Western blot - Anti-Angiogenin antibody (ab95389)All lanes : Anti-Angiogenin antibody (ab95389) at 1 µg/ml
Lane 1 : Lung (Human) Tissue Lysate
Lane 2 : Human liver tissue lysate - total protein (ab29889)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 17 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted?
Additional bands at: 125 kDa, 160 kDa, 23 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-Angiogenin antibody (ab95389)ICC/IF image of ab95389 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab95389 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References (1)
ab95389 has been referenced in 1 publication.
- Pan SC et al. Preliminary Assessment of Burn Depth by Paper-Based ELISA for the Detection of Angiogenin in Burn Blister Fluid-A Proof of Concept. Diagnostics (Basel) 10:N/A (2020). ELISA ; Human . PubMed: 32120826
Images
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Western blot - Anti-Angiogenin antibody (ab95389)All lanes : Anti-Angiogenin antibody (ab95389) at 1 µg/ml
Lane 1 : Lung (Human) Tissue Lysate
Lane 2 : Human liver tissue lysate - total protein (ab29889)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 17 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted?
Additional bands at: 125 kDa, 160 kDa, 23 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
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Immunocytochemistry/ Immunofluorescence - Anti-Angiogenin antibody (ab95389)
ICC/IF image of ab95389 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab95389 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
