Anti-RGS2 antibody (ab36561)
Key features and details
- Rabbit polyclonal to RGS2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RGS2 antibody
See all RGS2 primary antibodies -
Description
Rabbit polyclonal to RGS2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Dog
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Immunogen
Synthetic peptide corresponding to Human RGS2 aa 150 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab36560) -
Positive control
- ICC/IF: SKNSH cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab36561 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB (2) ICC/IF Notes Application notesICC/IF: Use at a concentration of 1 µg/ml.
IHC-P: Use at a dilution of 1/250 for 1 hr.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 24 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.Target
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Function
Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form (PubMed:11063746, PubMed:19478087). Plays a role in the regulation of blood pressure in response to signaling via G protein-coupled receptors and GNAQ. Plays a role in regulating the constriction and relaxation of vascular smooth muscle (By similarity). Binds EIF2B5 and blocks its activity, thereby inhibiting the translation of mRNA into protein (PubMed:19736320). -
Tissue specificity
Expressed in acute myelogenous leukemia (AML) and in acute lymphoblastic leukemia (ALL). -
Sequence similarities
Contains 1 RGS domain. -
Post-translational
modificationsPhosphorylated by protein kinase C. Phosphorylation by PRKG1 leads to activation of RGS2 activity. -
Cellular localization
Cell membrane. Mitochondrion and Cell membrane. Cytoplasm. Nucleus, nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 5997 Human
- Entrez Gene: 19735 Mouse
- Entrez Gene: 84583 Rat
- Omim: 600861 Human
- SwissProt: P41220 Human
- SwissProt: O08849 Mouse
- SwissProt: Q9JHX0 Rat
- Unigene: 78944 Human
see all -
Alternative names
- Basic helix-loop-helix phosphoprotein G0S8 antibody
- Cell growth inhibiting protein 31 antibody
- Cell growth-inhibiting gene 31 protein antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-RGS2 antibody (ab36561)ab36561 staining RGS2 in SKNSH cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab36561 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Western blot - Anti-RGS2 antibody (ab36561)Anti-RGS2 antibody (ab36561) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
Additional bands at: 51 kDa (possible cross reactivity) -
Western blot - Anti-RGS2 antibody (ab36561)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab36561 has been referenced in 10 publications.
- Ihlow J et al. Low Expression of RGS2 Promotes Poor Prognosis in High-Grade Serous Ovarian Cancer. Cancers (Basel) 14:N/A (2022). PubMed: 36230542
- Xing Y et al. Serum Exosomes Derived from Irritable Bowel Syndrome Patient Increase Cell Permeability via Regulating miR-148b-5p/RGS2 Signaling in Human Colonic Epithelium Cells. Gastroenterol Res Pract 2021:6655900 (2021). PubMed: 34221007
- Damasceno S et al. Top Common Differentially Expressed Genes in the Epileptogenic Nucleus of Two Strains of Rodents Susceptible to Audiogenic Seizures: WAR and GASH/Sal. Front Neurol 11:33 (2020). PubMed: 32117006
- Wang C et al. Downregulation of regulator of G protein signaling 2 expression in breast invasive carcinoma of no special type: Clinicopathological associations and prognostic relevance. Oncol Lett 15:213-220 (2018). PubMed: 29391880
- Linder A et al. Analysis of regulator of G-protein signalling 2 (RGS2) expression and function during prostate cancer progression. Sci Rep 8:17259 (2018). PubMed: 30467386
- Bouvet GF et al. DNA Methylation Regulates RGS2-induced S100A12 Expression in Airway Epithelial Cells. Am J Respir Cell Mol Biol 59:601-613 (2018). PubMed: 29944393
- Mather Q et al. A novel protein expression signature differentiates benign lipomas from well-differentiated liposarcomas. Mol Clin Oncol 7:315-321 (2017). PubMed: 28781809
- de Souza EE et al. Human Nek7-interactor RGS2 is required for mitotic spindle organization. Cell Cycle 14:656-67 (2015). PubMed: 25664600
- de Souza EE et al. Characterization of the human NEK7 interactome suggests catalytic and regulatory properties distinct from those of NEK6. J Proteome Res 13:4074-90 (2014). PubMed: 25093993
- Subramaniam S et al. Distinct transcriptional networks in quiescent myoblasts: a role for Wnt signaling in reversible vs. irreversible arrest. PLoS One 8:e65097 (2013). WB ; Mouse . PubMed: 23755177
Images
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Western blot - Anti-RGS2 antibody (ab36561)Anti-RGS2 antibody (ab36561) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
Additional bands at: 51 kDa (possible cross reactivity)
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Immunocytochemistry/ Immunofluorescence - Anti-RGS2 antibody (ab36561)ICC/IF image of ab36561 stained human SH-SY5Y cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab36561, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). -
Western blot - Anti-RGS2 antibody (ab36561)
