Anti-ID4 antibody [EPR22323-36] (ab220166)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22323-36] to ID4
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-ID4 antibody [EPR22323-36]
See all ID4 primary antibodies -
Description
Rabbit monoclonal [EPR22323-36] to ID4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human testis and ovary tissue lysates; Mouse testis and ovary tissue lysates; Rat testis and ovary tissue lysates; 3T3-L1, NIH/3T3, 293T and F9 whole cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22323-36 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-ID4 antibody [EPR22323-36] (ab220166) at 1/5000 dilution
Lane 1 : Human testis tissue lysate
Lane 2 : Human ovary tissue lysate
Lane 3 : Human liver tissue lysate
Lane 4 : Mouse testis tissue lysate
Lane 5 : Mouse ovary tissue lysate
Lane 6 : Mouse liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: liver (PMID:8139914).
Exposure time: 3 minutes.
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All lanes : Anti-ID4 antibody [EPR22323-36] (ab220166) at 1/1000 dilution
Lane 1 : Rat testis tissue lysate
Lane 2 : Rat ovary tissue lysate
Lane 3 : Rat liver tissue lysate
Lane 4 : 3T3-L1 (mouse embryonic fibroblast) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 6 : 293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 7 : F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: liver(PMID:8139914).
Exposure time: Lanes 1-3: 3 minutes; Lanes 4-5: 70 seconds; Lanes 6-7: 15 seconds.
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