Anti-ID4 antibody [EPR22323-36] (ab220166)
![Anti-ID4 antibody [EPR22323-36] (ab220166)](https://www.abcam.com/ps/products/220/ab220166/Images/ab220166-391295-anti-id4-antibody-epr2232336-western-blot-human-testis-tissue-lysate-20-ug.jpg "Anti-ID4 antibody [EPR22323-36] (ab220166)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22323-36] to ID4
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-ID4 antibody [EPR22323-36]
See all ID4 primary antibodies -
Description
Rabbit monoclonal [EPR22323-36] to ID4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
-
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Human testis and ovary tissue lysates; Mouse testis and ovary tissue lysates; Rat testis and ovary tissue lysates; 3T3-L1, NIH/3T3, 293T and F9 whole cell lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22323-36 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-ID4 antibody [EPR22323-36] (ab220166)All lanes : Anti-ID4 antibody [EPR22323-36] (ab220166) at 1/5000 dilution
Lane 1 : Human testis tissue lysate
Lane 2 : Human ovary tissue lysate
Lane 3 : Human liver tissue lysate
Lane 4 : Mouse testis tissue lysate
Lane 5 : Mouse ovary tissue lysate
Lane 6 : Mouse liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: liver (PMID:8139914).
Exposure time: 3 minutes.
-
![Western blot - Anti-ID4 antibody [EPR22323-36] (ab220166)](https://www.abcam.com/ps/products/220/ab220166/Images/ab220166-391296-anti-id4-antibody-epr2232336-western-blot-rat-testis-tissue-lysate-20-ug.jpg)
Western blot - Anti-ID4 antibody [EPR22323-36] (ab220166)All lanes : Anti-ID4 antibody [EPR22323-36] (ab220166) at 1/1000 dilution
Lane 1 : Rat testis tissue lysate
Lane 2 : Rat ovary tissue lysate
Lane 3 : Rat liver tissue lysate
Lane 4 : 3T3-L1 (mouse embryonic fibroblast) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 6 : 293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 7 : F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: liver(PMID:8139914).
Exposure time: Lanes 1-3: 3 minutes; Lanes 4-5: 70 seconds; Lanes 6-7: 15 seconds.
-
![Anti-ID4 antibody [EPR22323-36] (ab220166)](https://www.abcam.com/ps/products/220/ab220166/Images/ab220166-3-benefits-of-recombinant-antibodies.png)
Anti-ID4 antibody [EPR22323-36] (ab220166)
