Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Septin 2 antibody [EPR12123] (ab179436)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12123] to Septin 2
Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt
Reacts with: Human

Product name

Anti-Septin 2 antibody [EPR12123]
See all Septin 2 primary antibodies
Description

Rabbit monoclonal [EPR12123] to Septin 2
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human Septin 2 aa 250-350 (Cysteine residue). The exact sequence is proprietary.
Database link: Q15019
Positive control
- Human fetal brain, U87-MG, Jurkat and MCF-7 lysates. Human hepatocellular carcinoma and pancreas tissues. MCF-7 and Jurkat cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR12123
Isotype

IgG
Research areas

Western blot - Anti-Septin 2 antibody [EPR12123] (ab179436)

All lanes : Anti-Septin 2 antibody [EPR12123] (ab179436) at 1/1000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : U87-MG lysate
Lane 3 : Jurkat lysate
Lane 4 : MCF-7 lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 41 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 2 antibody [EPR12123] (ab179436)

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Septin 2 with ab179436 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 2 antibody [EPR12123] (ab179436)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Septin 2 with ab179436 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Septin 2 antibody [EPR12123] (ab179436)

Immunofluorescent analysis of MCF-7 cells labeling Septin 2 with ab179436 at 1/50 dilution (red). DAPI nuclear staining (blue).
Flow Cytometry - Anti-Septin 2 antibody [EPR12123] (ab179436)

Flow cytometric analysis of permeabilized Jurkat cells labeling Septin 2 with ab179436 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
Immunoprecipitation - Anti-Septin 2 antibody [EPR12123] (ab179436)

Western blot analysis on immunoprecipitation pellet from (1) MCF-7 cell lysate or (2) 1XPBS (negative control) using ab179436 at a 1/10 dilution for IP, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Anti-Septin 2 antibody [EPR12123] (ab179436)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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