Anti-MGA antibody [EPR19854] (ab214814)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19854] to MGA
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse
Overview
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Product name
Anti-MGA antibody [EPR19854]
See all MGA primary antibodies -
Description
Rabbit monoclonal [EPR19854] to MGA -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Wild-type mouse embryonic stem (ES) cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19854 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-MGA antibody [EPR19854] (ab214814) at 1/1000 dilution
Lane 1 : Wild-type mouse embryonic stem (ES) cell lysate
Lane 2 : MGA knockout mouse embryonic stem (ES) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 328 kDa
Observed band size: 400 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The cell lysates were kindly provided by our collaborator Dr. Jinzhong Qin, Nanjing University MARC.
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