Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CAPZA1 antibody [EPR11210] (ab166892)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11210] to CAPZA1
  • Suitable for: WB, IHC-P, IP, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CAPZA1 antibody [EPR11210]
    See all CAPZA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR11210] to CAPZA1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human CAPZA1.

  • Positive control

    • HeLa, Jurkat, 293T and HepG2 whole cell lysate (ab7900); Human cervical carcinoma and Human muscle tissues; Jurkat cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11210

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-CAPZA1 antibody [EPR11210] (ab166892)
    Western blot - Anti-CAPZA1 antibody [EPR11210] (ab166892)
    All lanes : Anti-CAPZA1 antibody [EPR11210] (ab166892) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 33 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] (ab166892)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] (ab166892)

    ab166892 showing +ve staining in Human thyroid gland carcinoma.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] (ab166892)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] (ab166892)

    ab166892 showing +ve staining in Human urinary bladder carcinoma.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] (ab166892)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] (ab166892)

    ab166892 showing +ve staining in Human colonic adenocarcinoma.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] (ab166892)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] (ab166892)

    ab166892 showing +ve staining in Human normal brain.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-CAPZA1 antibody [EPR11210] (ab166892)
    Immunoprecipitation - Anti-CAPZA1 antibody [EPR11210] (ab166892)
    ab166892, at 1/1000 dilution, staining CAPZA1 in HepG2 cell lysate immunoprecipitated using ab166892 at 1/10.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] (ab166892)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] (ab166892)

    Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue labeling CAPZA1 with ab166892 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] (ab166892)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] (ab166892)

    Immunohistochemical analysis of paraffin embedded Human muscle tissue labeling CAPZA1 with ab166892 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-CAPZA1 antibody [EPR11210] (ab166892)
    Flow Cytometry - Anti-CAPZA1 antibody [EPR11210] (ab166892)
    Flow cytometric analysis of permeabilized Jurkat cells using ab166892 at a dilution of 1/10 (red) compared to a rabbit IgG (negative) (green).
  • Anti-CAPZA1 antibody [EPR11210] (ab166892)
    Anti-CAPZA1 antibody [EPR11210] (ab166892)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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