Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Anti-CAPZA1 antibody [EPR11210] (ab166892)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR11210] to CAPZA1
Suitable for: WB, IHC-P, IP, Flow Cyt
Reacts with: Human

Product name

Anti-CAPZA1 antibody [EPR11210]
See all CAPZA1 primary antibodies
Description

Rabbit monoclonal [EPR11210] to CAPZA1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human CAPZA1.
Positive control
- HeLa, Jurkat, 293T and HepG2 whole cell lysate (ab7900); Human cervical carcinoma and Human muscle tissues; Jurkat cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR11210
Isotype

IgG
Research areas

Western blot - Anti-CAPZA1 antibody [EPR11210] (ab166892)

All lanes : Anti-CAPZA1 antibody [EPR11210] (ab166892) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HepG2 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 33 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] (ab166892)

ab166892 showing +ve staining in Human thyroid gland carcinoma.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] (ab166892)

ab166892 showing +ve staining in Human urinary bladder carcinoma.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] (ab166892)

ab166892 showing +ve staining in Human colonic adenocarcinoma.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] (ab166892)

ab166892 showing +ve staining in Human normal brain.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-CAPZA1 antibody [EPR11210] (ab166892)

ab166892, at 1/1000 dilution, staining CAPZA1 in HepG2 cell lysate immunoprecipitated using ab166892 at 1/10.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] (ab166892)

Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue labeling CAPZA1 with ab166892 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] (ab166892)

Immunohistochemical analysis of paraffin embedded Human muscle tissue labeling CAPZA1 with ab166892 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry - Anti-CAPZA1 antibody [EPR11210] (ab166892)

Flow cytometric analysis of permeabilized Jurkat cells using ab166892 at a dilution of 1/10 (red) compared to a rabbit IgG (negative) (green).
Anti-CAPZA1 antibody [EPR11210] (ab166892)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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