Anti-Brachyury / Bry antibody [1H9A2] (ab140661)
![Anti-Brachyury / Bry antibody [1H9A2] (ab140661)](https://www.abcam.com/ps/products/140/ab140661/Images/ab140661-156032-anti-brachyury-bry-antibody-1h9a2-immunohistochemistry.jpg "Anti-Brachyury / Bry antibody [1H9A2] (ab140661)")
Key features and details
- Mouse monoclonal [1H9A2] to Brachyury / Bry
- Suitable for: WB, IHC-P, Flow Cyt, ELISA
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-Brachyury / Bry antibody [1H9A2]
See all Brachyury / Bry primary antibodies -
Description
Mouse monoclonal [1H9A2] to Brachyury / Bry -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ELISA Recombinant fragmentFlow Cyt HumanIHC-P HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human Brachyury/ Bry aa 257-309. Purified recombinant fragment, corresponding to amino acids 257-309 of Human Brachyury/ Bry, expressed in E. Coli.
Sequence:PANPHPQFGGALSLPSTHSCDRYPTLRSHRSSPYPSPYAHRNNSPTYSDN SPA
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Positive control
- Recombinant Human Brachyury /Bry protein; HEK293 cells transfected with recombinant Human Brachyury / Bry fragment (aa 257-309); HeLa cells; Jurkat and Raji cell lysates; Human cervical cancer and rectum cancer tissues.
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General notes
This product was changed from ascites to supernatant. Lot no’s high than GR136145-8 are from Tissue Culture Supernatant
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS
Contains 0.5% protein stabilizer. -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
1H9A2 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brachyury / Bry antibody [1H9A2] (ab140661)Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling Brachyury / Bry with ab140661 at 1/200 dilution followed by DAB staining.
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![Western blot - Anti-Brachyury / Bry antibody [1H9A2] (ab140661)](https://www.abcam.com/ps/products/140/ab140661/Images/ab140661-156028-anti-brachyury-bry-antibody-1h9a2-western-blot.jpg)
Western blot - Anti-Brachyury / Bry antibody [1H9A2] (ab140661)All lanes : Anti-Brachyury / Bry antibody [1H9A2] (ab140661) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : HEK293 cell lysate, transfected with recombinant Human Brachyury / Bry fragment (aa 257-309)
Predicted band size: 47 kDa
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![Flow Cytometry - Anti-Brachyury / Bry antibody [1H9A2] (ab140661)](https://www.abcam.com/ps/products/140/ab140661/Images/ab140661-156030-anti-brachyury-bry-antibody-1h9a2-flow-cytometry.jpg)
Flow Cytometry - Anti-Brachyury / Bry antibody [1H9A2] (ab140661)Flow cytometric analysis of HeLa cells labeling Brachyury / Bry with ab140661 at 1/200 dilution (green); negative control (red). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brachyury / Bry antibody [1H9A2] (ab140661)](https://www.abcam.com/ps/products/140/ab140661/Images/ab140661-156031-anti-brachyury-bry-antibody-1h9a2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brachyury / Bry antibody [1H9A2] (ab140661)Immunohistochemical analysis of paraffin-embedded Human rectum cancer tissue labeling Brachyury / Bry with ab140661 at 1/200 dilution followed by DAB staining. -
![Western blot - Anti-Brachyury / Bry antibody [1H9A2] (ab140661)](https://www.abcam.com/ps/products/140/ab140661/Images/ab140661-156029-anti-brachyury-bry-antibody-1h9a2-western-blot.jpg)
Western blot - Anti-Brachyury / Bry antibody [1H9A2] (ab140661)Anti-Brachyury / Bry antibody [1H9A2] (ab140661) at 1/500 dilution + Recombinant Human Brachyury / Bry protein
Predicted band size: 47 kDa
Expected MW is 31.2 kDa. -
![ELISA - Anti-Brachyury / Bry antibody [1H9A2] (ab140661)](https://www.abcam.com/ps/products/140/ab140661/Images/ab140661-156026-anti-brachyury-bry-antibody-1h9a2-elisa.jpg)
ELISA - Anti-Brachyury / Bry antibody [1H9A2] (ab140661)ELISA analysis using ab140661. Antigen = Purified recombinant fragment, corresponding to amino acids 257-309 of Human Brachyury / Bry, expressed in E. Coli.
