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Developmental Biology Lineage specification Mesoderm

Anti-Brachyury / Bry antibody [6C12] (ab135254)

Anti-Brachyury / Bry antibody [6C12] (ab135254)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [6C12] to Brachyury / Bry
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Brachyury / Bry antibody [6C12]
    See all Brachyury / Bry primary antibodies
  • Description

    Mouse monoclonal [6C12] to Brachyury / Bry
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Purified recombinant fragment, corresponding to amino acids 218-352 of Human Brachyury/ Bry expressed in E.coli.

  • Positive control

    • Human Brachyury / Bry recombinant protein; Brachyury / Bry transfected HEK293 cell lysate; A549 cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS

    Protein stabilizer at 0.5%.
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    6C12
  • Isotype

    IgG1
  • Research areas

    • Stem Cells
    • Lineage Markers
    • Mesoderm
    • Stem Cells
    • Hematopoietic Progenitors
    • Intracellular Molecules
    • Cardiovascular
    • Heart
    • Cardiogenesis
    • Transcription factors/regulators
    • Developmental Biology
    • Lineage specification
    • Mesoderm

Images

  • Western blot - Anti-Brachyury / Bry antibody [6C12] (ab135254)
    Western blot - Anti-Brachyury / Bry antibody [6C12] (ab135254)
    Anti-Brachyury / Bry antibody [6C12] (ab135254) at 1/500 dilution + Human Brachyury / Bry recombinant protein.

    Predicted band size: 47 kDa



    Expected MW is 40.3 kDa.
  • Western blot - Anti-Brachyury / Bry antibody [6C12] (ab135254)
    Western blot - Anti-Brachyury / Bry antibody [6C12] (ab135254)
    All lanes : Anti-Brachyury / Bry antibody [6C12] (ab135254) at 1/500 dilution

    Lane 1 : HEK293 cell lysate, non-transfected
    Lane 2 : HEK293 cell lysate, transfected with Brachyury / Bry (amino acids 218-352)-hIgGFc.

    Predicted band size: 47 kDa

  • Flow Cytometry - Anti-Brachyury / Bry antibody [6C12] (ab135254)
    Flow Cytometry - Anti-Brachyury / Bry antibody [6C12] (ab135254)
    Flow cytometric analysis of A549 cells labelling Brachyury-Bry with ab135254 at 1/200 dilution (green) compared with a negative control (red).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Brachyury / Bry antibody [6C12] (ab135254)

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    Anti-Brachyury / Bry antibody (ab20680)

    Applications: ICC/IF, IHC-P, WB

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    Anti-Brachyury / Bry antibody (ab167337)

    Applications: ICC/IF, WB

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    Anti-Brachyury / Bry antibody [EPR18113] (ab209665)

    Applications: Flow Cyt, ICC/IF, IHC - Wmt, IHC-P, IP, WB

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    Anti-Brachyury / Bry antibody [1H9A2] (ab140661)

    Applications: ELISA, Flow Cyt, IHC-P, WB

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    Anti-Brachyury / Bry antibody [EPR18113] - BSA and Azide free (ab236023)

    Applications: Flow Cyt, ICC/IF, IHC - Wmt, IHC-P, IP, WB

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