Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Glypican 3 antibody [EPR10641] - BSA and Azide free (ab221498)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10641] to Glypican 3 - BSA and Azide free
  • Suitable for: WB, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Glypican 3 antibody [EPR10641] - BSA and Azide free
    See all Glypican 3 primary antibodies

  • Description

    Rabbit monoclonal [EPR10641] to Glypican 3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. The exact sequence is proprietary.
    Database link: P51654

  • Positive control

    • HepG2 cell lysate; Human fetal liver lysate; HepG2 cells.

  • General notes

    Ab221498 is the carrier-free version of ab174851. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab221498 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-Glypican 3 antibody [EPR10641] - BSA and Azide free (ab221498)
    Western blot - Anti-Glypican 3 antibody [EPR10641] - BSA and Azide free (ab221498)

    This data was developed using ab174851, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)

    Lane 2: GPC3 knockout HAP1 whole cell lysate (20 µg)

    Lane 3: HepG2 whole cell lysate (20 µg)

    Lanes 1 - 3: Merged signal (red and green). Green - ab174851 observed at 70 kDa. Red - loading control, ab130007, observed at 125 kDa.

    ab174851 was shown to recognize Glypican 3 in wild-type HAP1 cells as signal was lost at the expected MW in GPC3 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GPC3 knockout samples were subjected to SDS-PAGE. ab174851 and ab130007 (Mouse anti-vinculin loading control) were incubated overnight at 4°C at 1/1000 and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-Glypican 3 antibody [EPR10641] - BSA and Azide free (ab221498)
    Flow Cytometry - Anti-Glypican 3 antibody [EPR10641] - BSA and Azide free (ab221498)
    This data was developed using ab174851, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized HepG2 cells labeling Glypican 3 using ab174851 at 1/10 dilution (red) or a rabbit IgG negative (green).
  • Western blot - Anti-Glypican 3 antibody [EPR10641] - BSA and Azide free (ab221498)
    Western blot - Anti-Glypican 3 antibody [EPR10641] - BSA and Azide free (ab221498)
    All lanes : Anti-Glypican 3 antibody [EPR10641] (ab174851) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : Human fetal liver lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 66 kDa



    This data was developed using ab174851, the same antibody clone in a different buffer formulation.

     

  • Anti-Glypican 3 antibody [EPR10641] - BSA and Azide free (ab221498)
    Anti-Glypican 3 antibody [EPR10641] - BSA and Azide free (ab221498)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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