Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] - BSA and Azide free (ab208789)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5479] to Glutamate Receptor 1 (AMPA subtype) - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] - BSA and Azide free
    See all Glutamate Receptor 1 (AMPA subtype) primary antibodies

  • Description

    Rabbit monoclonal [EPR5479] to Glutamate Receptor 1 (AMPA subtype) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human, Mouse and Rat brain lysates.

  • General notes

    Ab208789 is the carrier-free version of ab109450. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab208789 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] - BSA and Azide free (ab208789)
    Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] - BSA and Azide free (ab208789)
    Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450) at 1/2000 dilution (purified) + Human brain lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 102 kDa



    This WB data was generated using the same anti-Gluatamate Receptor 1 (AMPA subtype) antibody clone, EPR5479, in a different buffer formulation (cat# ab109450).

    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] - BSA and Azide free (ab208789)
    Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] - BSA and Azide free (ab208789)

    ab109450 (purified) at 1/20 immunoprecipitating Glutamate Receptor 1 in Mouse Brain whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/1,500) was used for detection. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109450).

  • OI-RD Scanning - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] - BSA and Azide free (ab208789)
    OI-RD Scanning - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] - BSA and Azide free (ab208789)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109450).

  • Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] - BSA and Azide free (ab208789)
    Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] - BSA and Azide free (ab208789)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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