Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (ab32436)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E308] to Glutamate Receptor 1 (AMPA subtype)
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308]
    See all Glutamate Receptor 1 (AMPA subtype) primary antibodies

  • Description

    Rabbit monoclonal [E308] to Glutamate Receptor 1 (AMPA subtype)

  • Host species

    Rabbit

  • Specificity

    This antibody is specific for Glutamate Receptor 1 (AMPA subtype).

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Glutamate Receptor 1 (AMPA subtype) aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • WB: SH-SY5Y cell lysate. IHC: human brain tissue. IP: SH-SY5Y cell lysate

  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (ab32436)
    Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (ab32436)
    Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (ab32436) at 1/1000 dilution + SH-SY5Y cell lysate.

    Predicted band size: 101 kDa
    Observed band size: 106 kDa
    why is the actual band size different from the predicted?

  • Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (ab32436)
    Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (ab32436)

    Purified ab32436 at 1/50 dilution (2µg) immunoprecipitating Glutamate Receptor 1 (AMPA subtype) in SH-SY5Y whole cell lysate.
    Lane 1 (input): SY-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab32436 + SH-SY5Y whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32436 in SH-SY5Y whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 106 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (ab32436)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (ab32436)
    Ab32436, at a 1/50 dilution, staining Glutamate Receptor 1 (AMPA subtype) in paraffin embedded human brain tissue by Immunohistochemistry.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (ab32436)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (ab32436) Image from van Vuurden DG et al., PLoS One. 2009 Jun 18;4(6):e5953. Fig 2.; doi:10.1371/journal.pone.0005953; June 18, 2009, PLoS ONE 4(6): e5953.
    Immunohistochemical analysis of Human hippocampus tissue, staining Glutamate Receptor 1 (AMPA subtype) with ab32436.

    Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6). Samples were blocked with 10% normal goat serum for 30 minutes before incubating with primary antibody (1/20) for 2 hours. A biotinylated anti-rabbit IgG was used as the secondary antibody and staining was detected using DAB.
  • Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (ab32436)
    Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (ab32436)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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