Anti-Glutamate Receptor 1 (AMPA subtype) (phospho T840) antibody (ab12108)
Key features and details
- Rabbit polyclonal to Glutamate Receptor 1 (AMPA subtype) (phospho T840)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Glutamate Receptor 1 (AMPA subtype) (phospho T840) antibody
See all Glutamate Receptor 1 (AMPA subtype) primary antibodies -
Description
Rabbit polyclonal to Glutamate Receptor 1 (AMPA subtype) (phospho T840) -
Host species
Rabbit -
Specificity
ab12108 detects two bands >100kDa in WB. The higher MW band is probably a phospho protein as the signal is decreased when the blot is incubated with phosphatase prior to ab12108 (data not shown). However, this upper band is not GluR1 protein, as it is only detected by ab12108 and not by other GluR1 antibodies; it is therefore an unknown protein which is bound to specifically by ab12108. -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
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Positive control
- This antibody gave a positive signal in Mouse Brain tissue lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab12108 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseAll applications HumanApplication Abreviews Notes WB (1) Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 102 kDa.Can be blocked with Recombinant Human Glutamate Receptor 1 (AMPA subtype) protein (ab112297).Notes WB
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 102 kDa.Can be blocked with Recombinant Human Glutamate Receptor 1 (AMPA subtype) protein (ab112297).Target
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Function
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. -
Tissue specificity
Widely expressed in brain. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily. -
Post-translational
modificationsPalmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis. -
Cellular localization
Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2 promotes cell surface expression. - Information by UniProt
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Database links
- Entrez Gene: 2890 Human
- Entrez Gene: 14799 Mouse
- Omim: 138248 Human
- SwissProt: P42261 Human
- SwissProt: P23818 Mouse
- Unigene: 519693 Human
- Unigene: 737709 Human
- Unigene: 4920 Mouse
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Alternative names
- GLUR 1 antibody
- GLUR A antibody
- AMPA 1 antibody
see all
Images
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Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho T840) antibody (ab12108)This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United Kingdomab12108 detects 2 bands over 100kDa by Western Blot. Both of these bands are blocked by the immunizing phospho peptide (lane 4), but not by the non-modified peptide (lane 5). However the immunoprecipitation data (below) demonstrates that the upper band (at ~120kDa) is unlikely to be GluR1 and therefore is an unknown protein.
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Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho T840) antibody (ab12108)This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United KingdomLanes 1 & 4 & 7-8 : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho T840) antibody (ab12108) at 3 µg/ml
Lane 3 : Preimmune mouse IgG antibody
Lane 5 : Blot reprobed with GluR1 mouse antibody
Lane 1 : Mouse brain extract that has not been through IP protocol
Lanes 2 & 6 : Blank
Lane 3 : Control IP for mouse IgG
Lane 4 : Mouse brain extract following IP for GluR1
Lane 7 : Input lane reprobed with GluR1 mouse antibody
Lane 8 : IP lane reprobed with GluR1 mouse antibody
Performed under reducing conditions.
Predicted band size: 102 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?
Additional bands at: 120 kDa (possible cross reactivity), 55 kDa (possible IgG)Lane 1 (input) has two bands >100kDa as seen in the WB for ab12108, this lane is loaded with brain extract that has not been through the IP protocol. The higher MW band in the Lane 1 (input) is probably a phospho protein as the signal is decreased when the blot is incubated with phosphatase prior to ab12108 (data not shown). However, this upper band is not GluR1 protein, as it is only detected by ab12108 and not by other GluR1 antibodies; it is therefore an unknown protein which is bound to specifically by ab12108.
Lane 3 is a control IP with mouse IgG - this is the result of using a 'pre-immune' mouse IgG antibody to see if anything is pulled down from the brain extract independent of the GluR1 antibody. The ~55kDa bands in this lane correspond to IgGs.
Lane 4 is a WB for ab12108 following IP for GluR1. This lane should only contain GluR1 (band at ~100kDa) and anything bound to it (plus IgG). The single band at the right size for GluR1 in Lane 4 is therefore likely
Protocols
References (5)
ab12108 has been referenced in 5 publications.
- Guzman RE et al. Involvement of ClC-3 chloride/proton exchangers in controlling glutamatergic synaptic strength in cultured hippocampal neurons. Front Cell Neurosci 8:143 (2014). WB ; Mouse . PubMed: 24904288
- Gray EE et al. Inhibitory interactions between phosphorylation sites in the C terminus of a-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid-type glutamate receptor GluA1 subunits. J Biol Chem 289:14600-11 (2014). WB, IP ; Mouse . PubMed: 24706758
- Williams LE & Featherstone DE Regulation of hippocampal synaptic strength by glial xCT. J Neurosci 34:16093-102 (2014). IHC ; Mouse . PubMed: 25429150
- Szabo ST et al. Glutamate receptors as targets of protein kinase C in the pathophysiology and treatment of animal models of mania. Neuropharmacology 56:47-55 (2009). WB ; Mouse . PubMed: 18789340
- Delgado JY et al. NMDA receptor activation dephosphorylates AMPA receptor glutamate receptor 1 subunits at threonine 840. J Neurosci 27:13210-21 (2007). WB, ICC/IF ; Mouse . PubMed: 18045915
Images
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Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho T840) antibody (ab12108) This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United Kingdomab12108 detects 2 bands over 100kDa by Western Blot. Both of these bands are blocked by the immunizing phospho peptide (lane 4), but not by the non-modified peptide (lane 5). However the immunoprecipitation data (below) demonstrates that the upper band (at ~120kDa) is unlikely to be GluR1 and therefore is an unknown protein.
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Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho T840) antibody (ab12108) This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United KingdomLanes 1 & 4 & 7-8 : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho T840) antibody (ab12108) at 3 µg/ml
Lane 3 : Preimmune mouse IgG antibody
Lane 5 : Blot reprobed with GluR1 mouse antibody
Lane 1 : Mouse brain extract that has not been through IP protocol
Lanes 2 & 6 : Blank
Lane 3 : Control IP for mouse IgG
Lane 4 : Mouse brain extract following IP for GluR1
Lane 7 : Input lane reprobed with GluR1 mouse antibody
Lane 8 : IP lane reprobed with GluR1 mouse antibody
Performed under reducing conditions.
Predicted band size: 102 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?
Additional bands at: 120 kDa (possible cross reactivity), 55 kDa (possible IgG)Lane 1 (input) has two bands >100kDa as seen in the WB for ab12108, this lane is loaded with brain extract that has not been through the IP protocol. The higher MW band in the Lane 1 (input) is probably a phospho protein as the signal is decreased when the blot is incubated with phosphatase prior to ab12108 (data not shown). However, this upper band is not GluR1 protein, as it is only detected by ab12108 and not by other GluR1 antibodies; it is therefore an unknown protein which is bound to specifically by ab12108.
Lane 3 is a control IP with mouse IgG - this is the result of using a 'pre-immune' mouse IgG antibody to see if anything is pulled down from the brain extract independent of the GluR1 antibody. The ~55kDa bands in this lane correspond to IgGs.
Lane 4 is a WB for ab12108 following IP for GluR1. This lane should only contain GluR1 (band at ~100kDa) and anything bound to it (plus IgG). The single band at the right size for GluR1 in Lane 4 is therefore likely
