Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸ 10 µl 130 665 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4646] to FLI1
Suitable for: IHC-P, WB
Reacts with: Mouse, Human, Recombinant fragment

Product name

Anti-FLI1 antibody [EPR4646]
See all FLI1 primary antibodies
Description

Rabbit monoclonal [EPR4646] to FLI1
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

Recombinant fragment

See all applications and species data
Immunogen

Synthetic peptide within Human FLI1 aa 350-450. The exact sequence is proprietary.
(Peptide available as ab187137)
Positive control
- U937, Jurkat, Raw264.7 and HL-60 cell lysates; Human Ewing sarcoma tissue; FLI1 recombinant protein
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IHC-P	Human
WB	Human Recombinant fragment

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4646
Isotype

IgG
Research areas
- Cell Biology
- Apoptosis
- Nucleus
- Other
- Cancer
- Cell Death
- Apoptosis
- Nucleus
- Other

Western blot - Anti-FLI1 antibody [EPR4646] (ab133485)

Anti-FLI1 antibody [EPR4646] (ab133485) at 1/1000 dilution (purified) + Jurkat cell lysate at 10 µg

Secondary
HRP goat anti-rabbit at 1/1000 dilution

Predicted band size: 51 kDa
Observed band size: 51 kDa

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLI1 antibody [EPR4646] (ab133485)

Immunohistochemical staining of paraffin embedded human lung with purified ab133485 at a working dilution of 1 in 700. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
Western blot - Anti-FLI1 antibody [EPR4646] (ab133485)

Anti-FLI1 antibody [EPR4646] (ab133485) at 1/200 dilution (unpurified) + Jurkat cell lysate at 10 µg

Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 51 kDa
Observed band size: 51 kDa

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-FLI1 antibody [EPR4646] (ab133485)

All lanes : Anti-FLI1 antibody [EPR4646] (ab133485) at 1/1000 dilution (unpurified)

Lane 1 : U937 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Raw264.7 cell lysate
Lane 4 : HL-60 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 51 kDa
Western blot - Anti-FLI1 antibody [EPR4646] (ab133485)

All lanes : Anti-FLI1 antibody [EPR4646] (ab133485) at 1/1000 dilution (unpurified)

Lane 1 : FLI1 recombinant protein
Lane 2 : ERG
recombinant protein

Lysates/proteins at 0.01 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 51 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLI1 antibody [EPR4646] (ab133485)

Immunohistochemical staining of paraffin embedded human lung with unpurified ab133485 at a working dilution of 1 in 140. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLI1 antibody [EPR4646] (ab133485)

Immunohistochemical analysis of paraffin-embedded Human Ewing sarcoma tissue labelled with unpurified ab133485 at 1/250 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-FLI1 antibody [EPR4646] (ab133485)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com