Anti-FLI1 antibody (ab90583)
Key features and details
- Rabbit polyclonal to FLI1
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-FLI1 antibody
See all FLI1 primary antibodies -
Description
Rabbit polyclonal to FLI1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide corresponding to Human FLI1 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab101301) -
Positive control
- This antibody gave a positive signal in MEF1 and NIH3T3 whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab90583 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseAll applications RatChickenCowHumanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 51 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 51 kDa).Target
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Function
Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'. -
Involvement in disease
Defects in FLI1 are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving FLI1 is found in patients with Erwing sarcoma. Translocation t(11;22)(q24;q12) with EWSR1. -
Sequence similarities
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Contains 1 PNT (pointed) domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2313 Human
- Entrez Gene: 14247 Mouse
- Entrez Gene: 315532 Rat
- Omim: 193067 Human
- SwissProt: Q01543 Human
- SwissProt: P26323 Mouse
- Unigene: 504281 Human
- Unigene: 258908 Mouse
see all -
Alternative names
- ERGB transcription factor antibody
- Ewing Sarcoma breakpoint region 2 antibody
- EWSR 2 antibody
see all
Images
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Western blot - Anti-FLI1 antibody (ab90583)All lanes : Anti-FLI1 antibody (ab90583) at 1 µg/ml
Lane 1 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
Additional bands at: 19 kDa, 31 kDa, 52 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Datasheets and documents
References (0)
ab90583 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-FLI1 antibody (ab90583)All lanes : Anti-FLI1 antibody (ab90583) at 1 µg/ml
Lane 1 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
Additional bands at: 19 kDa, 31 kDa, 52 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
