All lanes : Anti-BDNF antibody [3B2] (ab205067) at 2.5 µg/ml

Lane 1 : Human hippocampus with MES
Lane 2 : Rat hippocampus with MES
Lane 3 : Mouse hippocampus with MES

Secondary
All lanes : Goat anti-Mouse800 Licor at 1/10000 dilution

Predicted band size: 15 kDa

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel ran at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for 1 hour before incubation with ab205067 (2.5 microgram /mL) overnight at 4°C.  Antibody binding was detected using infrared labelled goat anti-mouse IgG (green) at a 1:10,000 dilution for 1 hour at room temperature.

Immunofluorescent analysis of U2OS cells labeling BDNF with ab205067 at 0.33 µg/ml, followed by Goat anti-mouse AlexaFluor488 secondary antibody. For nuclear staining DAPI was used. A: proBDNF-expressing U2OS cells; B. Negative control (non-transfected U2OS cells).

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cerebral cortex tissue sections from Alzheimer's disease patients labeling BDNF with ab205067 at 5 µg/ml, followed by peroxidase secondary detection and DAB staining. A: BDNF staining by ab205067; B.Negative staining without primary antibody. Sections were counterstained with toluidine blue.

Anti-BDNF antibody [3B2] (ab205067) at 5 µg/ml + 10 µl of CHO cell culture supernatant containing BDNF at 10 µl

Secondary
HRP-conjugated Goat anti-Mouse IgG

Predicted band size: 15 kDa