Anti-BDNF antibody (ab226843)
Key features and details
- Rabbit polyclonal to BDNF
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-BDNF antibody
See all BDNF primary antibodies -
Description
Rabbit polyclonal to BDNF -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF RatIHC-P MouseRatWB MouseRatHumanRecombinant fragment
-
Immunogen
Synthetic peptide within Human BDNF (internal sequence). The exact sequence is proprietary. Carrier-protein conjugated synthetic peptide.
Database link: P23560 -
Positive control
- IHC-P: Adult mouse retina tissue, Rat brain tissue. WB: Mouse and rat brain tissue extract,Transfected (+) 293T whole cell extracts ICC/IF: DIV9 rat E18 primary cortical neuron cells.
-
General notes
For BDNF, multiple WB bands are possible and expected. The human protein has 5 isoforms (precursors: 28 – 37 kDa) and can be glycosylated (Uniprot: P23560). The mature form is expected at ~14 kDa (monomer) and the dimer at ~28 kDa.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-BDNF antibody (ab226843)All lanes : Anti-BDNF antibody (ab226843) at 1/500 dilution (4? overnight)
Lane 1 : Mouse brain
Lane 2 : Rat brain
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Additional bands at: 17 kDa (possible mature (processed) protein)12% SDS-PAGE.
Running: 80V, 15min; 140V, 40 min. Transfer: Semi-dry, 18 V, 60 min (NC embrane). Blocking: 5% non-fat milk in TBST, RT, 60 minutes. Washing: 5 ml TBST, 4 x 5 min. ECL exposure.
-
Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody (ab226843)Immunocytochemistry/ Immunofluorescence analysis of DIV9 rat E18 primary cortical neuron cells labeling BDNF with ab226843 at 1:500 (green). Cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes. Cells were counterstained with DAPI (blue) and a Tau antibody diluted at 1/500 (yellow).
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody (ab226843)Immunohistochemical analysis of paraffin-embedded rat brain tissue labeling BDNF with ab226843 at 1/500 dilution at 4°C overnight. Antigen Retrieval:Citrate buffer, pH 6.0, 15 min (Cuisinart Electric Pressure Cooker #EPC-1200, choose "high pressure"). Endogenous peroxidase blocking: 3% H2O2, RT, 30min. Blocking:1.5% goat serum (dilute goat serum by 1xPBS), RT, 30 minutes. Secondary antibody incubation: ABC HRP Kit (Rabbit IgG), 1:200, RT, 30min. Washing: PBS, 2 x 5 mins. Chromogen system: DAB.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody (ab226843)Immunohistochemical analysis of paraffin-embedded adult mouse retina tissue labeling BDNF with ab226843 at 1/250 dilution (green). Counter nuclear stained with DAPI (blue) and a beta III Tubulin antibody diluted at 1/500 (red).
-
Western blot - Anti-BDNF antibody (ab226843)All lanes : Anti-BDNF antibody (ab226843) at 1/1000 dilution
Lane 1 : Non-transfected (–) 293T whole cell extracts
Lane 2 : Transfected (+) 293T whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody15% SDS-PAGE
