Anti-RTN3/HAP antibody (ab264314)
Key features and details
- Rabbit polyclonal to RTN3/HAP
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RTN3/HAP antibody
See all RTN3/HAP primary antibodies -
Description
Rabbit polyclonal to RTN3/HAP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human RTN3/HAP aa 750-800. The exact sequence is proprietary. AAU81930.1
Database link: O95197 -
Positive control
- IHC-P: Human ovarian carcinoma tissue. WB: HeLa whole cell lysate. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RTN3/HAP antibody (ab264314)All lanes : Anti-RTN3/HAP antibody (ab264314) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 113 kDa
Exposure time: 30 seconds
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Immunoprecipitation - Anti-RTN3/HAP antibody (ab264314)RTN3/HAP was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264314 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264314 at 0.4 µg/ml.
Lane 1: ab264314 IP in HeLa whole cell lysate.
Lane 2: Control IgG.Exposure time: 3 secs.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTN3/HAP antibody (ab264314)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovarian carcinoma tissue labeling RTN3/HAP with ab264314 at 1/200 dilution.
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
