Antibodies, Proteins, Kits and Reagents for Life Science

335 040 ₸ Product size

100 µl 335 040 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1320Y] to NGF
Suitable for: ICC/IF, WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-NGF antibody [EP1320Y]
See all NGF primary antibodies
Description

Rabbit monoclonal [EP1320Y] to NGF
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide within Human NGF aa 200-300. The exact sequence is proprietary.
(Peptide available as ab154297)
Positive control
- WB: HeLa cell lysate and Human fetal brain tissue lysate; mouse thyroid lysate; rat thyroid lysate IHC-P: Human brain, human cerebral cortex. ICC/IF: U87-MG cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP1320Y
Isotype

IgG
Research areas

Western blot - Anti-NGF antibody [EP1320Y] (ab52918)

All lanes : Anti-NGF antibody [EP1320Y] (ab52918) at 1/1000 dilution (purified)

Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal thymus lysate
Lane 3 : HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 27 kDa
Observed band size: 32 kDa
why is the actual band size different from the predicted?

Blocking Buffer: 5% NFDM/TBST
Dilution Buffer: 5% NFDM/TBST
Western blot - Anti-NGF antibody [EP1320Y] (ab52918)

Anti-NGF antibody [EP1320Y] (ab52918) at 1/1000 dilution (purified) + Mouse thyroid lysate at 20 µg

Secondary
HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 27 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?

Blocking Buffer: 5% NFDM/TBST
Dilution Buffer: 5% NFDM/TBST
Western blot - Anti-NGF antibody [EP1320Y] (ab52918)

Anti-NGF antibody [EP1320Y] (ab52918) at 1/2000 dilution (purified) + Rat thyroid lysate at 20 µg

Secondary
HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 27 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?

Blocking Buffer: 5% NFDM/TBST
Dilution Buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NGF antibody [EP1320Y] (ab52918)

Immunohistochemical staining of paraffin embedded human cerebral cortex with purified ab52918 at a working dilution of 1 in 250. The secondary antibody used is ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Immunocytochemistry/ Immunofluorescence - Anti-NGF antibody [EP1320Y] (ab52918)

Immunofluorescence staining of U87-MG cells with purified ab52918 at a working dilution of 1 in 300, counter-stained with DAPI. Tubulin was stained with mouse anti-tubulin at a dilution of 1/1000 (ab7291) and Alexa Fluor^® 594 goat anti-mouse at a dilution of 1/500 (ab150120) . The secondary antibody was ab150077 Alexa Fluor^® 488 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in the bottom middle and right hand panels - for the first negative control, purified ab52918 was used at a dilution of 1/200 followed by an Alexa Fluor^® 555 goat anti-mouse antibody at a dilution of 1/500 and for the second negative control mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab15007) were used.
Western blot - Anti-NGF antibody [EP1320Y] (ab52918)

Anti-NGF antibody [EP1320Y] (ab52918) at 1/500 dilution (unpurified) + fetal thyroid tissue at 10 µg

Secondary
Goat anti rabbit IgG HRP antibody at 1/2000 dilution

Predicted band size: 27 kDa
Observed band size: 27 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NGF antibody [EP1320Y] (ab52918)

Immunohistochemical staining of paraffin embedded human brain using unpurified ab52918 at 1/50-1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NGF antibody [EP1320Y] (ab52918) This image is courtesy of an anonymous Abreview.

Immunohistochemical staining of NGF mouse tissue sections (formalin/ PFA-fixed paraffin-embedded tissue sections) with unpurified ab52918. The sections were formaldehyde fixed, subjected to heat mediated antigen retrieval and blocked for 10 minutes at 25°C. The primary antibody was diluted 1/50 and incubated with the sample for 1 hour at 25°C. An HRP polymer anti-rabbit IgG system was used undiluted, as the secondary antibody.

See Abreview
Anti-NGF antibody [EP1320Y] (ab52918)

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