Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

Order now and get it on Tuesday March 16, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1318Y] to NGF
Suitable for: WB, Flow Cyt
Reacts with: Rat, Human

Product name

Anti-NGF antibody [EP1318Y]
See all NGF primary antibodies
Description

Rabbit monoclonal [EP1318Y] to NGF
Host species

Rabbit
Specificity

This antibody recognizes pro-NGF.
Tested Applications & Species

Application Species
Flow Cyt
Human

WB
Rat

Human

See all applications and species data
Immunogen

Synthetic peptide within Human NGF aa 1-100 (N terminal). The exact sequence is proprietary.
Positive control
- Human pro-NGF recombinant protein
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
Flow Cyt	Human
WB	Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP1318Y
Isotype

IgG
Research areas

Flow Cytometry - Anti-NGF antibody [EP1318Y] (ab68151)

Overlay histogram showing SH-SY5Y cells stained with ab68151 (red line) at 1/280 dilution. The cells were fixed with 2% paraformaldehyde. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (black line) was rabbit monoclonal IgG used under the same conditions. Cells also incubated without primary antibody and secondary antibody (blue line)
Western blot - Anti-NGF antibody [EP1318Y] (ab68151)

Anti-NGF antibody [EP1318Y] (ab68151) at 1/1000 dilution + Rat brain lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

Predicted band size: 27 kDa
Western blot - Anti-NGF antibody [EP1318Y] (ab68151)

Anti-NGF antibody [EP1318Y] (ab68151) at 1/1000 dilution + Human fetal brain lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/1000 dilution

Predicted band size: 27 kDa
Western blot - Anti-NGF antibody [EP1318Y] (ab68151)

All lanes : Anti-NGF antibody [EP1318Y] (ab68151) at 1/5000 dilution (unpurified)

Lane 1 : 10ng human pro-NGF recombinant protein
Lane 2 : 5ng human pro-NGF recombinant protein
Lane 3 : 1ng human pro-NGF recombinant protein
Lane 4 : 0.5ng human pro-NGF recombinant protein

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 27 kDa
Observed band size: 27 kDa
Additional bands at: 13 kDa, 20 kDa. We are unsure as to the identity of these extra bands.
Anti-NGF antibody [EP1318Y] (ab68151)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com