Anti-NGF antibody (ab6199)
Key features and details
- Rabbit polyclonal to NGF
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NGF antibody
See all NGF primary antibodies -
Description
Rabbit polyclonal to NGF -
Host species
Rabbit -
Specificity
Less than 1% cross-reactivity against recombinant human Brain Derived Neurotrophic Factor, Neurotrophin 3 and Neurotrophin 4/5 by ELISA. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Does not react with: Cow -
Immunogen
Full length native protein (purified) corresponding to Mouse NGF.
Database link: P01139 -
Positive control
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General notes
This antibody has been shown to be useful for a variety of techniques and its specificity has been demonstrated by immunoblot.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Constituent: PBS
pH: 7.2-7.6 without preservatives. -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NGF antibody (ab6199)All lanes : Anti-NGF antibody (ab6199) at 5 µg/ml
Lane 1 : rhNGF protein at 100 µg
Lane 2 : Mouse salivary gland tissue lysate at 50 µgab6199 detects a strong band at 13 kDa consistent with the expected molecular weight of mature NGF monomer.
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Western blot - Anti-NGF antibody (ab6199) -
Immunocytochemistry/ Immunofluorescence - Anti-NGF antibody (ab6199)ICC/IF image of ab6199 stained MEF1 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6199, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
