Anti-Synaptophysin antibody (ab14692)
Key features and details
- Rabbit polyclonal to Synaptophysin
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-Synaptophysin antibody
See all Synaptophysin primary antibodies -
Description
Rabbit polyclonal to Synaptophysin -
Host species
Rabbit -
Specificity
The antibody does not cross-react other synaptic vesicle proteins such as synaptotagmin, GAP45, and SNAP25 protiens. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human Synaptophysin aa 41-62.
Sequence:FATCGSYSGELQLSVDCANKTE
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Positive control
- IHC: Rat and mouse brain. WB: Mouse forebrain extract.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
The buffer includes stabilizing proteins and glycerol. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody (ab14692)
Immunohistochemical analysis of formaldehyde-fixed, paraffin-embedded mouse brain sections labelling Synaptophysin with ab14692 at a dilution of 1/100 In IHC blocking Buffer. DAB (brown_ staining and Hematoxylin QS (blue) counterstain was used. 20x magnfication of Leica DM4000.
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Western blot - Anti-Synaptophysin antibody (ab14692) This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United KingdomSynaptophysin antibody (ab14692) western blot on mouse forebrain extract (10µg protein/lane). The blot was exposed for 10 minutes to autoradiographic film. Ladder markers from 7-194 kDa.
Lane 1 = 1/500 ab14692
Lane 2 = 1/200 ab14692 -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody (ab14692)Immunohistochemical analysis of formaldehyde-fixed, paraffin-embedded rat brain sections labelling Synaptophysin with ab14692 at a dilution of 1/100 In IHC blocking Buffer. DAB (brown_ staining and Hematoxylin QS (blue) counterstain was used. 20x magnfication of Leica DM4000.
