Anti-Fbx32 antibody (ab74023)
Key features and details
- Rabbit polyclonal to Fbx32
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-Fbx32 antibody
See all Fbx32 primary antibodies -
Description
Rabbit polyclonal to Fbx32 -
Host species
Rabbit -
Specificity
This antibody is only batch tested in WB and therefore we can only guarantee it for this application. Customers have successfully used this antibody in IHC and ICC but we have not tested it's performance in these applications. If you would like an antibody that is tested in ICC, we would recommend our recombinant antibody ab168372. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Pig, Zebrafish
-
Immunogen
Synthetic peptide within Human Fbx32 aa 300 to the C-terminus (internal sequence) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
(Peptide available asab74022) -
Positive control
- WB: Human, mouse and rat skeletal muscle tissue lysate. IHC-P: Human heart tissue. IHC-Fr: Rat muscle tissue. ICC/IF: Rat astrocyte cells.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab74023 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 2 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). Target
-
Function
Substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1. -
Tissue specificity
Specifically expressed in cardiac and skeletal muscle. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 F-box domain. - Information by UniProt
-
Database links
- Entrez Gene: 513776 Cow
- Entrez Gene: 114907 Human
- Entrez Gene: 67731 Mouse
- Entrez Gene: 733657 Pig
- Entrez Gene: 171043 Rat
- Omim: 606604 Human
- SwissProt: Q2KHT6 Cow
- SwissProt: Q969P5 Human
see all -
Alternative names
- 4833442G10Rik antibody
- AI430017 antibody
- Atrogin 1 antibody
see all
Images
-
Western blot - Anti-Fbx32 antibody (ab74023)All lanes : Anti-Fbx32 antibody (ab74023) at 2 µg/ml
Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 2 : Mouse skeletal muscle tissue lysate
Lane 3 : Rat skeletal muscle tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Additional bands at: 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 15 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fbx32 antibody (ab74023)This image is courtesy of an abreview submitted by Antibody Solutions Ltd.Paraffin-embedded human heart tissue stained for Fbx32 with ab74023 (1/2,000 dilution) in immunohistochemical analysis.
Tissue underwent fixation in formaldehyde, peroxidase blocking, protein blocking and heat mediated antigen retrieval. The secondary antibody was goat anti-rabbit conjugated to HRP. For further experimental details please refer to abreview.
-
Immunohistochemistry (Frozen sections) - Anti-Fbx32 antibody (ab74023)This image is courtesy of an Abreview submitted by Ruma Raha-ChowdhuryFrozen sectioned rat muscle tissue stained for Fbx32 with ab74023 (1/200 dilution) (green) in immunohistochemical analysis.
Tissue was fixed with 4% PFA overnight. Samples were then blocked with 10% donkey serum for 1 hour at 24°C followed by incubation with ab74023 for 24 hours at 4°C. A pig anti-rabbit Alexa Fluor488® conjugate was used as secondary antibody at a 1/1,000 dilution. Nuclei were stained using the standard Hoesch method and are depicted in blue.
-
Immunocytochemistry/ Immunofluorescence - Anti-Fbx32 antibody (ab74023)This image is courtesy of an Abreview submitted by Ruma Raha-Chowdhuryab74023 staining Fbx32 in cultured rat astrocytes by ICC/IF at a 1/500 dilution (green).
The cultured astrocytes were fixed with paraformaldehyde and blocked with 10% donkey serum for 30 minutes at 24°C. Cells were then stained with ab74023 in 0.3% TritonX with 0.1% PBS and 10% donkey serum for 4.5 hours at 24°C. An Alexa Fluor-488® donkey anti-rabbit polyclonal antibody at 1/1,000 was used as the secondary antibody. Nuclei were stained with 1.43µM Hoechst and can be observed in blue. Fbx32 expressed in the newly born astrocytes but does not co-localise with mature astrocytes.
Protocols
References (18)
ab74023 has been referenced in 18 publications.
- Su J et al. The rat model of COPD skeletal muscle dysfunction induced by progressive cigarette smoke exposure: a pilot study. BMC Pulm Med 20:74 (2020). PubMed: 32293377
- Adhikary S et al. FGF-2 targets sclerostin in bone and myostatin in skeletal muscle to mitigate the deleterious effects of glucocorticoid on musculoskeletal degradation. Life Sci 229:261-276 (2019). PubMed: 31082400
- Ahmad N et al. MicroRNA-672-5p Identified during Weaning Reverses Osteopenia and Sarcopenia in Ovariectomized Mice. Mol Ther Nucleic Acids 14:536-549 (2019). PubMed: 30769134
- Cella PS et al. Creatine supplementation in Walker-256 tumor-bearing rats prevents skeletal muscle atrophy by attenuating systemic inflammation and protein degradation signaling. Eur J Nutr N/A:N/A (2019). PubMed: 30806774
- Li Z et al. LncIRS1 controls muscle atrophy via sponging miR-15 family to activate IGF1-PI3K/AKT pathway. J Cachexia Sarcopenia Muscle 10:391-410 (2019). PubMed: 30701698
Images
-
Western blot - Anti-Fbx32 antibody (ab74023)All lanes : Anti-Fbx32 antibody (ab74023) at 2 µg/ml
Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 2 : Mouse skeletal muscle tissue lysate
Lane 3 : Rat skeletal muscle tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Additional bands at: 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 15 minutes
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fbx32 antibody (ab74023) This image is courtesy of an abreview submitted by Antibody Solutions Ltd.
Paraffin-embedded human heart tissue stained for Fbx32 with ab74023 (1/2,000 dilution) in immunohistochemical analysis.
Tissue underwent fixation in formaldehyde, peroxidase blocking, protein blocking and heat mediated antigen retrieval. The secondary antibody was goat anti-rabbit conjugated to HRP. For further experimental details please refer to abreview.
-
Immunohistochemistry (Frozen sections) - Anti-Fbx32 antibody (ab74023) This image is courtesy of an Abreview submitted by Ruma Raha-Chowdhury
Frozen sectioned rat muscle tissue stained for Fbx32 with ab74023 (1/200 dilution) (green) in immunohistochemical analysis.
Tissue was fixed with 4% PFA overnight. Samples were then blocked with 10% donkey serum for 1 hour at 24°C followed by incubation with ab74023 for 24 hours at 4°C. A pig anti-rabbit Alexa Fluor488® conjugate was used as secondary antibody at a 1/1,000 dilution. Nuclei were stained using the standard Hoesch method and are depicted in blue.
-
Immunocytochemistry/ Immunofluorescence - Anti-Fbx32 antibody (ab74023) This image is courtesy of an Abreview submitted by Ruma Raha-Chowdhury
ab74023 staining Fbx32 in cultured rat astrocytes by ICC/IF at a 1/500 dilution (green).
The cultured astrocytes were fixed with paraformaldehyde and blocked with 10% donkey serum for 30 minutes at 24°C. Cells were then stained with ab74023 in 0.3% TritonX with 0.1% PBS and 10% donkey serum for 4.5 hours at 24°C. An Alexa Fluor-488® donkey anti-rabbit polyclonal antibody at 1/1,000 was used as the secondary antibody. Nuclei were stained with 1.43µM Hoechst and can be observed in blue. Fbx32 expressed in the newly born astrocytes but does not co-localise with mature astrocytes.
