Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TrkB (phospho Y706) antibody [EPR19399] - BSA and Azide free (ab251239)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19399] to TrkB (phospho Y706) - BSA and Azide free
  • Suitable for: Dot blot, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-TrkB (phospho Y706) antibody [EPR19399] - BSA and Azide free
    See all TrkB primary antibodies

  • Description

    Rabbit monoclonal [EPR19399] to TrkB (phospho Y706) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251239 is the carrier-free version of ab197072. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251239 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-TrkB (phospho Y706) antibody [EPR19399] - BSA and Azide free (ab251239)
    Western blot - Anti-TrkB (phospho Y706) antibody [EPR19399] - BSA and Azide free (ab251239)
    All lanes : Anti-TrkB (phospho Y706) antibody [EPR19399] (ab197072) at 1/1000 dilution

    Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (wild type) with Myc-tag
    Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (wild type) with Myc-tag starved for 4 hours, then treated with 50 ng/ml BDNF for 10 minutes
    Lane 3 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (phospho Y706A mutant) with Myc-tag
    Lane 4 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (phospho Y706A mutant) with Myc-tag starved for 4 hours, then treated with 50 ng/ml BDNF for 10 minutes

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 92 kDa
    Observed band size: 140 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 second


    This data was developed using ab197072, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The observed MW is higher than the predicted one due to the glycosylation. The expression pattern is consistent with the published papers (PMID: 23115189 and 20064930).

  • Dot Blot - Anti-TrkB (phospho Y706) antibody [EPR19399] - BSA and Azide free (ab251239)
    Dot Blot - Anti-TrkB (phospho Y706) antibody [EPR19399] - BSA and Azide free (ab251239)
    This data was developed using ab197072, the same antibody clone in a different buffer formulation.Dot blot analysis of TrkB (phospho Y706) labeled with ab197072 at 1/1000 dilution. Lane 1: TrkB (phospho Y706) phospho peptide; Lane 2: TrkB Non-phospho peptide; Lane 3: TrkA (phospho Y680) phospho peptide; Lane 4: TrkA Non-phospho peptide. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution was used as secondary antibody. Blocking and diluting buffer: 5% NFDM/TBST. Exposure time: 3 minutes. Note: ab197072 has weak cross reactivity with TrkA (phospho Y680) according to the dot blot data.
  • Anti-TrkB (phospho Y706) antibody [EPR19399] - BSA and Azide free (ab251239)
    Anti-TrkB (phospho Y706) antibody [EPR19399] - BSA and Azide free (ab251239)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.