Anti-TrkB (phospho Y706) antibody [EPR19399] - BSA and Azide free (ab251239)
![Anti-TrkB (phospho Y706) antibody [EPR19399] - BSA and Azide free (ab251239)](https://www.abcam.com/ps/products/251/ab251239/Images/ab251239-403471-anti-trkb-phospho-y706-antibody-epr19399-bsa-and-azide-free-western-blot.jpg "Anti-TrkB (phospho Y706) antibody [EPR19399] - BSA and Azide free (ab251239)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19399] to TrkB (phospho Y706) - BSA and Azide free
- Suitable for: Dot blot, WB
- Reacts with: Human
Overview
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Product name
Anti-TrkB (phospho Y706) antibody [EPR19399] - BSA and Azide free
See all TrkB primary antibodies -
Description
Rabbit monoclonal [EPR19399] to TrkB (phospho Y706) - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251239 is the carrier-free version of ab197072. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251239 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR19399 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TrkB (phospho Y706) antibody [EPR19399] - BSA and Azide free (ab251239)All lanes : Anti-TrkB (phospho Y706) antibody [EPR19399] (ab197072) at 1/1000 dilution
Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (wild type) with Myc-tag
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (wild type) with Myc-tag starved for 4 hours, then treated with 50 ng/ml BDNF for 10 minutes
Lane 3 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (phospho Y706A mutant) with Myc-tag
Lane 4 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (phospho Y706A mutant) with Myc-tag starved for 4 hours, then treated with 50 ng/ml BDNF for 10 minutes
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 92 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondThis data was developed using ab197072, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
The observed MW is higher than the predicted one due to the glycosylation. The expression pattern is consistent with the published papers (PMID: 23115189 and 20064930).
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![Dot Blot - Anti-TrkB (phospho Y706) antibody [EPR19399] - BSA and Azide free (ab251239)](https://www.abcam.com/ps/products/251/ab251239/Images/ab251239-1-anti-trkb-phospho-y706-antibody-epr19399-bsa-and-azide-free-dot-blot.jpg)
Dot Blot - Anti-TrkB (phospho Y706) antibody [EPR19399] - BSA and Azide free (ab251239)This data was developed using ab197072, the same antibody clone in a different buffer formulation.Dot blot analysis of TrkB (phospho Y706) labeled with ab197072 at 1/1000 dilution. Lane 1: TrkB (phospho Y706) phospho peptide; Lane 2: TrkB Non-phospho peptide; Lane 3: TrkA (phospho Y680) phospho peptide; Lane 4: TrkA Non-phospho peptide. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution was used as secondary antibody. Blocking and diluting buffer: 5% NFDM/TBST. Exposure time: 3 minutes. Note: ab197072 has weak cross reactivity with TrkA (phospho Y680) according to the dot blot data. -
![Anti-TrkB (phospho Y706) antibody [EPR19399] - BSA and Azide free (ab251239)](https://www.abcam.com/ps/products/251/ab251239/Images/ab251239-3-benefits-of-recombinant-antibodies.png)
Anti-TrkB (phospho Y706) antibody [EPR19399] - BSA and Azide free (ab251239)
