Anti-Cytokeratin 17 antibody - Cytoskeleton Marker (ab53707)
Key features and details
- Rabbit polyclonal to Cytokeratin 17 - Cytoskeleton Marker
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cytokeratin 17 antibody - Cytoskeleton Marker
See all Cytokeratin 17 primary antibodies -
Description
Rabbit polyclonal to Cytokeratin 17 - Cytoskeleton Marker -
Host species
Rabbit -
Specificity
ab53707 detects endogenous levels of total Cytokeratin 17 protein. -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide derived from human Cytokeratin 17.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab53707 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody - Cytoskeleton Marker (ab53707)ab53707 at 1/50 dilution staining Cytokeratin 17 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
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Western blot - Anti-Cytokeratin 17 antibody - Cytoskeleton Marker (ab53707)All lanes : Anti-Cytokeratin 17 antibody - Cytoskeleton Marker (ab53707) at 1/500 dilution
Lane 1 : HuvEc cell extract
Lane 2 : HuvEc cell extract with immunising peptide
Predicted band size: 48 kDa
Observed band size: 48 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 17 antibody - Cytoskeleton Marker (ab53707)ICC/IF image of ab53707 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53707, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
