Anti-p75 NGF Receptor antibody [5C4B9] (ab86768)
Key features and details
- Mouse monoclonal [5C4B9] to p75 NGF Receptor
- Suitable for: WB
- Reacts with: Mouse
- Isotype: n/a
Overview
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Product name
Anti-p75 NGF Receptor antibody [5C4B9]
See all p75 NGF Receptor primary antibodies -
Description
Mouse monoclonal [5C4B9] to p75 NGF Receptor -
Host species
Mouse -
Specificity
Please note that, despite having used an immunogen that is 100% homologous with the human and rat p75 NGF receptor proteins, Abcam could not get this antibody to detect p75 NGF receptor in human or rat tissue lysates. Abcam would welcome feedback from customers that have used ab86768 in either of these species! -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in the following mouse tissue lysates: Brain; Spinal Cord; Heart.
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General notes
This antibody clone is manufactured by Abcam.
This antibody is available as an azide-free product (see ab175435).
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
5C4B9 -
Research areas
Images
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All lanes : Anti-p75 NGF Receptor antibody [5C4B9] (ab86768) at 5 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Spinal Cord (Mouse) Tissue Lysate
Lane 3 : Heart (Mouse) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 75 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
Please note that, despite having used an immunogen that is 100% homologous with the human and rat p75 NGF receptor proteins, Abcam could not get this antibody to detect p75 NGF receptor in human or rat tissue lysates. Abcam would welcome feedback from customers that have used ab86768 in either of these species!