Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-p75 NGF Receptor antibody [EP1039Y] - BSA and Azide free (ab256584)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1039Y] to p75 NGF Receptor - BSA and Azide free
  • Suitable for: WB, IP, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-p75 NGF Receptor antibody [EP1039Y] - BSA and Azide free
    See all p75 NGF Receptor primary antibodies

  • Description

    Rabbit monoclonal [EP1039Y] to p75 NGF Receptor - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Rat
    ICC/IF
    Rat
    IHC-P
    Human
    IP
    Rat
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: PC-12 cells. IHC-P: Human tonsil tissue; Mouse uterus tissue. IP: PC-12 cell lysate. Flow Cyt: PC-12 cells.

  • General notes

    ab256584 is the carrier-free version of ab52987. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256584 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody [EP1039Y] - BSA and Azide free (ab256584)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody [EP1039Y] - BSA and Azide free (ab256584)

    Purified ab52987 staining p75 NGF receptor in paraffin embedded Human tonsil tissue sections by Immunohistochemistry. Antigen retrieval was by heat mediation using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody at 3.3μg/ml. A ready to use Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on germinal centre of human tonsil.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52987).

  • Flow Cytometry - Anti-p75 NGF Receptor antibody [EP1039Y] - BSA and Azide free (ab256584)
    Flow Cytometry - Anti-p75 NGF Receptor antibody [EP1039Y] - BSA and Azide free (ab256584)

    Flow Cytometry analysis of PC-12 (rat adrenal gland pheochromocytoma) cells labeling p75 NGF Receptor with unpurified ab52987 at 1/60 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor®488) at 1/2000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52987).

  • Immunoprecipitation - Anti-p75 NGF Receptor antibody [EP1039Y] - BSA and Azide free (ab256584)
    Immunoprecipitation - Anti-p75 NGF Receptor antibody [EP1039Y] - BSA and Azide free (ab256584)

    Lane 1 (input): PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate 10μg
    Lane 2 (+): PC-12 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52987 in PC-12 whole cell lysate

    ab52987 immunoprecipitating p75 NGF receptor in PC-12 whole cell lysates. For western blotting, primary antibody used was ab52987 at 1.6 μg/ml. Ab131366 VeriBlot for IP (HRP) was used for detection at 1/1000 dilution. Capture antibody was used at 1:40 dilution (2μg in 0.35mg lysates).

    Blocking and diluting buffer: 5% NFDM/TBST.

    Exposure: 10 seconds

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52987).

  • Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody [EP1039Y] - BSA and Azide free (ab256584)
    Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody [EP1039Y] - BSA and Azide free (ab256584)

    Purified ab52987 staining p75 NGF receptor in PC-12 (rat adrenal gland pheochromocytoma) by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% TritonX-100. Samples were incubated with primary antibody at 3.9 µg/ml. An AlexaFluor®488 Goat anti-Rabbit was used as the secondary antibody at 2 µg/ml. DAPI was used as a nuclear counterstain. Confocal image showing cytoplasmic and Membranous staining in PC-12 cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52987).

  • Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody [EP1039Y] - BSA and Azide free (ab256584)
    Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody [EP1039Y] - BSA and Azide free (ab256584)

    ICC/IF image of ab52987 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52987, 1 µg/mL) overnight at 4oC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluo® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.4 µM.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52987).

  • Flow Cytometry - Anti-p75 NGF Receptor antibody [EP1039Y] - BSA and Azide free (ab256584)
    Flow Cytometry - Anti-p75 NGF Receptor antibody [EP1039Y] - BSA and Azide free (ab256584)

    Flow Cytometry analysis of PC-12 (rat adrenal gland pheochromocytoma) cells labeling p75 NGF Receptor with unpurified ab52987 at 1/60 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor®488) at 1/2000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52987).

  • OI-RD Scanning - Anti-p75 NGF Receptor antibody [EP1039Y] - BSA and Azide free (ab256584)
    OI-RD Scanning - Anti-p75 NGF Receptor antibody [EP1039Y] - BSA and Azide free (ab256584)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52987).

  • Anti-p75 NGF Receptor antibody [EP1039Y] - BSA and Azide free (ab256584)
    Anti-p75 NGF Receptor antibody [EP1039Y] - BSA and Azide free (ab256584)

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