Anti-p75 NGF Receptor antibody (ab227509)
Key features and details
- Rabbit polyclonal to p75 NGF Receptor
- Suitable for: WB, IHC-P, IHC-Fr, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-p75 NGF Receptor antibody
See all p75 NGF Receptor primary antibodies -
Description
Rabbit polyclonal to p75 NGF Receptor -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IHC-Fr, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow
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Immunogen
Recombinant fragment within Human p75 NGF Receptor (internal sequence). The exact sequence is proprietary.
Database link: P08138 -
Positive control
- WB: NTERA-2 cl.D1 [NT2/D1], PC-3 and U-87 MG whole cell lysates; PC-12 whole cell extract; Mouse and rat brain tissue extracts. IHC-P: Rat brain tissue. IHC-Fr: Rat E13.5 brain tissue. ICC/IF: Culture rat E18 primary cortical neurons.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody (ab227509)
Paraffin-embedded rat brain tissue stained for p75 NGF Receptor with ab227509 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-p75 NGF Receptor antibody (ab227509)All lanes : Anti-p75 NGF Receptor antibody (ab227509) at 1/2000 dilution
Lane 1 : NTERA-2 cl.D1 [NT2/D1] (human malignant pluripotent embryonic carcinoma cell line) whole cell lysate
Lane 2 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
Lane 3 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 45 kDa7.5% SDS-PAGE gel.
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Western blot - Anti-p75 NGF Receptor antibody (ab227509)Anti-p75 NGF Receptor antibody (ab227509) at 1/2000 dilution + PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract at 30 µg
Predicted band size: 45 kDa10% SDS-PAGE gel.
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Western blot - Anti-p75 NGF Receptor antibody (ab227509)All lanes : Anti-p75 NGF Receptor antibody (ab227509) at 1/2000 dilution
Lane 1 : Mouse brain tissue extract
Lane 2 : Rat brain tissue extract
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 45 kDa10% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody (ab227509)4% paraformaldehyde-fixed rat E18 primary cortical neuron cells stained for p75 NGF Receptor (green) using ab227509 at 1/250 dilution in ICC/IF.
Counterstain: Beta Tubulin 3/ TUJ1, stained with beta Tubulin 3/ TUJ1 antibody (red). Nuclear counterstain: Fluoroshield with DAPI (blue).
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Immunohistochemistry (Frozen sections) - Anti-p75 NGF Receptor antibody (ab227509)Frozen section of rat E13.5 brain tissue stained for p75 NGF Receptor (green) using ab227509 at 1/250 dilution in immunohistochemical analysis.
Counterstain: Beta-Tubulin is detected with an anti-beta-Tubulin antibody (red). Nuclear counterstain: Fluroshield with DAPI (blue).
