Anti-p75 NGF Receptor antibody [NGFR5] (ab3125)
![Anti-p75 NGF Receptor antibody [NGFR5] (ab3125)](https://www.abcam.com/ps/products/3/ab3125/Images/ab3125-455389-Antip75-NGF-Receptor-antibody-Immunohistochemisty-Human-Testis.jpg "Anti-p75 NGF Receptor antibody [NGFR5] (ab3125)")
Key features and details
- Mouse monoclonal [NGFR5] to p75 NGF Receptor
- Suitable for: ICC, IHC-P
- Reacts with: Rat
- Isotype: IgG1
Overview
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Product name
Anti-p75 NGF Receptor antibody [NGFR5]
See all p75 NGF Receptor primary antibodies -
Description
Mouse monoclonal [NGFR5] to p75 NGF Receptor -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF RatWB Human
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Immunogen
NGFR from A875 melanoma cells.
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Epitope
Amino acids 1 - 160. -
Positive control
- IHC: Human testis tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituents: 0.0268% PBS, 0.2% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
NGFR5 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody [NGFR5] (ab3125)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue labelling p75 NGF Receptor with ab3125 at 2 µg/mL.
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![Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody [NGFR5] (ab3125)](https://www.abcam.com/ps/products/3/ab3125/Images/ab3125-7299-anti-p75-ngf-receptor-antibody-ngfr5-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody [NGFR5] (ab3125)ICC/IF image of ab3125 stained PC-12 (Rat adrenal gland pheochromocytoma cell line) cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3125, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
