Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸ 10 µl 113 243 ₸

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Anti-APOBEC3B antibody [EPR18138] (ab184990)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18138] to APOBEC3B
Suitable for: WB
Reacts with: Human

Product name

Anti-APOBEC3B antibody [EPR18138]
See all APOBEC3B primary antibodies
Description

Rabbit monoclonal [EPR18138] to APOBEC3B
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB:Human APOBEC3B fragment recombinant protein; HepG2, Jurkat and THP-1 whole cell lysates; Human fetal heart lysate; HepaRG-APOBEC3B tetracycline-inducible cell line; HCC1143 cell line infected with control (+) and APOBEC3B lentivirus (-); HEK293-APOBEC3B(+GFP) tetracycline-inducible cell line.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18138
Isotype

IgG
Research areas

Western blot - Anti-APOBEC3B antibody [EPR18138] (ab184990)

All lanes : Anti-APOBEC3B antibody [EPR18138] (ab184990) at 1/1000 dilution

Lane 1 : Human APOBEC3B fragment recombinant protein
Lane 2 : Human APOBEC3G fragment recombinant protein

Lysates/proteins at 0.01 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

Predicted band size: 46 kDa
Observed band size: 46 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-APOBEC3B antibody [EPR18138] (ab184990)

Anti-APOBEC3B antibody [EPR18138] (ab184990) at 1/1000 dilution + HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

Predicted band size: 46 kDa
Observed band size: 46 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-APOBEC3B antibody [EPR18138] (ab184990)

Anti-APOBEC3B antibody [EPR18138] (ab184990) at 1/2000 dilution + Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

Predicted band size: 46 kDa
Observed band size: 46 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-APOBEC3B antibody [EPR18138] (ab184990)

Anti-APOBEC3B antibody [EPR18138] (ab184990) at 1/1000 dilution + THP-1 (Human monocytic leukemia cell line) whole cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

Predicted band size: 46 kDa
Observed band size: 46 kDa

Exposure time: 1 minute

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-APOBEC3B antibody [EPR18138] (ab184990)

Anti-APOBEC3B antibody [EPR18138] (ab184990) at 1/1000 dilution + Human fetal heart lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 46 kDa
Observed band size: 46 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-APOBEC3B antibody [EPR18138] (ab184990)

All lanes : Anti-APOBEC3B antibody [EPR18138] (ab184990) at 1/10000 dilution

Lane 1 : HepaRG-APOBEC3B tetracycline-inducible cell line (+/-)
Lane 2 : HCC1143 cell line infected with control (+) and APOBEC3B lentivirus (-)
Lane 3 : HEK293-APOBEC3B(+GFP) tetracycline-inducible cell line (+/-)

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG (H+L) conjugated to HRP at 1/10000 dilution

Predicted band size: 46 kDa
Observed band size: 35,70 kDa
why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST.

Dilution buffer: 1% NFDM/TBST.

Exposure time: Panel A: 20 s; Panel B: 1 min.

Figure provided by Dr. Jenni Nikkila. Gene Function Lab at The Institute of Cancer Research, London. HEK293-APOBEC3B(+GFP) tetracycline-inducible cell line provide by Dr Reuben Harris, University of Minnesota, Minneapolis - cited in Akre, M.K, G.J. Starrett, J.S. Quist, N.A. Temiz, M.A. Carpenter, A.N.J. Tutt, A. Grigoriadis & R.S. Harris (2016) Mutation processes in 293-based clones overexpressing the DNA cytosine deaminase APOBEC3B. PLoS One11(5):e0155391. doi: 10.1371/journal.pone.0155391.

The 35 kDa band represents a known splice variant of APOBEC3B.
Anti-APOBEC3B antibody [EPR18138] (ab184990)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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