Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673-33] - BSA and Azide free (ab250479)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13673-33] to Heterogeneous Nuclear Ribonucleoprotein (A1-like) - BSA and Azide free
  • Suitable for: ICC, IHC-P, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673-33] - BSA and Azide free
    See all Heterogeneous Nuclear Ribonucleoprotein (A1-like) primary antibodies

  • Description

    Rabbit monoclonal [EPR13673-33] to Heterogeneous Nuclear Ribonucleoprotein (A1-like) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IHC-P, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250479 is the carrier-free version of ab181337. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250479 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673-33] - BSA and Azide free (ab250479)
    Western blot - Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673-33] - BSA and Azide free (ab250479)
    All lanes : Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673-33] (ab181337) at 1/10000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : K562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 34 kDa



    This data was developed using ab181337, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673-33] - BSA and Azide free (ab250479)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673-33] - BSA and Azide free (ab250479)
    This data was developed using ab181337, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human transitional cell carcinoma of bladder tissue sections labeling Heterogeneous Nuclear Ribonucleoprotein (A1-like) using ab181337 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673-33] - BSA and Azide free (ab250479)
    Immunocytochemistry - Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673-33] - BSA and Azide free (ab250479)
    This data was developed using ab181337, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of paraformaldehyde fixed A431 cells labeling Heterogeneous Nuclear Ribonucleoprotein (A1-like) using ab181337 at a 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) was used as the secondary at a 1/200 dilution.
  • Flow Cytometry - Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673-33] - BSA and Azide free (ab250479)
    Flow Cytometry - Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673-33] - BSA and Azide free (ab250479)
    This data was developed using ab181337, the same antibody clone in a different buffer formulation.Flow Cytometry analysis of K562 cells labeling Heterogeneous Nuclear Ribonucleoprotein (A1-like) using ab181337 at a 1/20 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.
  • Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673-33] - BSA and Azide free (ab250479)
    Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673-33] - BSA and Azide free (ab250479)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.