Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] - BSA and Azide free (ab250868)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10891(2)] to MYH6 + Slow Skeletal Myosin Heavy chain - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] - BSA and Azide free

  • Description

    Rabbit monoclonal [EPR10891(2)] to MYH6 + Slow Skeletal Myosin Heavy chain - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The immunogen used for this product shares 78%,76%,76%,76%,72%homology with Myosin 2 1 3 8 4, respectively. Cross-reactivity with these proteins has not been confirmed experimentally.

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: His tagged recombinant Human Myosin-7 and 6 protein, mouse and rat heart lysates. IHC: Human skeletal muscle

  • General notes

    ab250868 is the carrier-free version of ab185967.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] - BSA and Azide free (ab250868)
    Western blot - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] - BSA and Azide free (ab250868)
    Lane 1 : Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (ab185967) at 1/1000 dilution
    Lane 2 : Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) at 1/1000 dilution (His Tag)

    All lanes : His tagged recombinant Human Myosin-7 protein(aa 1191-1335)

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 224 kDa



    ab185967 could recognize both Myosin 6 and 7 protein

  • Western blot - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] - BSA and Azide free (ab250868)
    Western blot - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] - BSA and Azide free (ab250868)
    Lane 1 : Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (ab185967) at 1/1000 dilution
    Lane 2 : Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) at 1/1000 dilution

    All lanes : His tagged recombinant Human Myosin-6 protein(aa 1193-1337)

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG,(H+L), Peroxidase conjugated)

    Predicted band size: 224 kDa



    ab185967 could recognize both Myosin 6 and 7 protein

  • Western blot - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] - BSA and Azide free (ab250868)
    Western blot - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] - BSA and Azide free (ab250868)
    All lanes : Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (ab185967) at 1/500 dilution

    Lane 1 : Human adult heart lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Rat heart lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti Rabbit IR680 at 1/10000 dilution

    Predicted band size: 224 kDa



    This data was developed using ab185967, the same antibody clone in a different buffer formulation.

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab185967 overnight at 4°C. Antibody binding was detected using Goat anti Rabbit IR680 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] - BSA and Azide free (ab250868)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] - BSA and Azide free (ab250868)

    This data was developed using ab185967, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue labeling Slow Skeletal Myosin Heavy chain with ab185967 at 1/2000 dilution. Counterstained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] - BSA and Azide free (ab250868)
    Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] - BSA and Azide free (ab250868)

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