Anti-Fast Myosin Skeletal Heavy chain antibody [MY-32] (ab51263)
![Anti-Fast Myosin Skeletal Heavy chain antibody [MY-32] (ab51263)](https://www.abcam.com/ps/products/51/ab51263/Images/ab51263-4-anti-fast-myosin-skeletal-heavy-chain-antibody-immunocytochemistry-ips-myocytes-human-my32.jpg "Anti-Fast Myosin Skeletal Heavy chain antibody [MY-32] (ab51263)")
Key features and details
- Mouse monoclonal [MY-32] to Fast Myosin Skeletal Heavy chain
- Suitable for: ICC
- Reacts with: Rabbit
- Isotype: IgG1
Overview
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Product name
Anti-Fast Myosin Skeletal Heavy chain antibody [MY-32]
See all Fast Myosin Skeletal Heavy chain primary antibodies -
Description
Mouse monoclonal [MY-32] to Fast Myosin Skeletal Heavy chain -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC RabbitIHC-P Rabbit
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Immunogen
Full length native protein (purified) corresponding to Rabbit Fast Myosin Skeletal Heavy chain. Purified rabbit muscle myosin.
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Positive control
- ICC: ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612).
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General notes
This product was changed from ascites to tissue culture supernatant on 18th October 2016. The following lot is from ascites and is still in stock as of 18th October 2016 (GR255796). Lot numbers higher than GR255796 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
May be used for detecting cross striated muscle differentiation in tumors.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
Purified from Tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
MY-32 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry - Anti-Fast Myosin Skeletal Heavy chain antibody [MY-32] (ab51263)
Immunofluorescence staining of Fast Myosin Skeletal Heavy chain using ab51263 in ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612), which were differentiated for 3 (left panel), 5 (middle panel) and 10 days (right panel) post induction.
The cells were fixed with 100% MeOH (5 min) and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab51263 at 5 µg/mL and ab6046, rabbit polyclonal to beta Tubulin, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150088, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown. Gamma is adjusted to 1.5 in all channels.
The antibody ab51263 gave comparable results using 4% formaldehyde fixation (10 min).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fast Myosin Skeletal Heavy chain antibody [MY-32] (ab51263)](https://www.abcam.com/ps/products/51/ab51263/Images/ab51263-448507-anti-fast-myosin-skeletal-Heavy-chain-antibody-immunohistochemistry-tongue-rabbit.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fast Myosin Skeletal Heavy chain antibody [MY-32] (ab51263)Immunohistochemistry analysis of Formalin-fixed, paraffin-embedded Rabbit tongue sections labeling Fast Myosin Skeletal Heavy chain with ab51263 at 20 μg/mL followed by Goat Anti-Mouse IgG (Fab specific)-FITC antibody. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fast Myosin Skeletal Heavy chain antibody [MY-32] (ab51263)](https://www.abcam.com/ps/products/51/ab51263/Images/ab51263-316965-anti-fast-myosin-skeletal-heavy-chain-antibody-my-32-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fast Myosin Skeletal Heavy chain antibody [MY-32] (ab51263) This image is courtesy of an abreview by Carl Hobbsab51263 staining Fast Myosin Skeletal Heavy chain in pig skeletal muscle tissue sections by Immunohistochemistry (IHC-P - Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/1000 in TBS/BSA/azide) for 2 hours at 21°C. A Biotin-conjugated goat anti-mouse IgG polyclonal (1/300) was used as the secondary antibody.
