Anti-Myosin light chain kinase/MLCK antibody (ab232949)
Key features and details
- Rabbit polyclonal to Myosin light chain kinase/MLCK
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Myosin light chain kinase/MLCK antibody
See all Myosin light chain kinase/MLCK primary antibodies -
Description
Rabbit polyclonal to Myosin light chain kinase/MLCK -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Sheep, Rabbit, Cow
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Immunogen
Recombinant fragment corresponding to Human Myosin light chain kinase/MLCK aa 1650-1900. (N-terminal His-tag and GST-tag; Expressed in E.coli).
Database link: Q15746 -
Positive control
- IHC-P: Human esophagus cancer and kidney tissues. WB: Human lung lysate; Rat prostate gland and bladder lysates; Recombinant human Myosin light chain kinase/MLCK protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab232949 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain kinase/MLCK antibody (ab232949)
Paraffin-embedded human esophagus cancer tissue stained for Myosin light chain kinase/MLCK antibody using ab232949 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain kinase/MLCK antibody (ab232949)Paraffin-embedded human kidney tissue stained for Myosin light chain kinase/MLCK antibody using ab232949 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Western blot - Anti-Myosin light chain kinase/MLCK antibody (ab232949)Anti-Myosin light chain kinase/MLCK antibody (ab232949) at 2 µg/ml + Recombinant human Myosin light chain kinase/MLCK protein
Secondary
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 211 kDa
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Western blot - Anti-Myosin light chain kinase/MLCK antibody (ab232949)All lanes : Anti-Myosin light chain kinase/MLCK antibody (ab232949) at 2 µg/ml
Lane 1 : Human lung lysate
Lane 2 : Rat prostate gland lysate
Lane 3 : Rat bladder lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 211 kDa
