Anti-Myosin light chain kinase/MLCK (phospho S1760) antibody (ab200809)
Key features and details
- Rabbit polyclonal to Myosin light chain kinase/MLCK (phospho S1760)
- Suitable for: WB
- Reacts with: Rabbit, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-Myosin light chain kinase/MLCK (phospho S1760) antibody
See all Myosin light chain kinase/MLCK primary antibodies -
Description
Rabbit polyclonal to Myosin light chain kinase/MLCK (phospho S1760) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB RabbitAfrican green monkey
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Immunogen
Synthetic peptide corresponding to Human Myosin light chain kinase/MLCK (phospho S1760). A phosphopeptide derived from the region of Human Myosin light chain kinase/ MLCK that contains serine 1760.
Database link: Q15746 -
Positive control
- KCl-treated rabbit muscle extracts; COS cells over-expressing rabbit smooth muscle short Myosin light chain kinase/MLCK, phosphorylated in vitro by PKA.
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General notes
Previously labelled as Myosin light chain kinase.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 0.1% BSA, 49% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab200809 has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Myosin light chain kinase/MLCK. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Myosin light chain kinase/MLCK (phospho S1760) antibody (ab200809)All lanes : Anti-Myosin light chain kinase/MLCK (phospho S1760) antibody (ab200809) at 1/1000 dilution
Lane 1 : Extracts of COS cells transfected with rabbit muscle Myosin light chain kinase/MLCK, unstimulated
Lane 2 : Extracts of COS cells transfected with rabbit muscle Myosin light chain kinase/MLCK phosphorylated in vitro by 0.05 µg of PKA
Lane 3 : Extracts of COS cells transfected with rabbit muscle Myosin light chain kinase/MLCK phosphorylated in vitro by 0.05 µg of PKA with the non-phosphopeptide corresponding to the phosphopeptide immunogen
Lane 4 : Extracts of COS cells transfected with rabbit muscle Myosin light chain kinase/MLCK phosphorylated in vitro by 0.05 µg of PKA with a generic phosphoserine-containing peptide
Lane 5 : Extracts of COS cells transfected with rabbit muscle Myosin light chain kinase/MLCK phosphorylated in vitro by 0.05 µg of PKA with the phosphopeptide immunogen
Lane 6 : Extracts of COS cells transfected with rabbit muscle Myosin light chain kinase/MLCK phosphorylated in vitro by 0.05 µg of PKA, treated with
lambda phophatase
Secondary
All lanes : goat F(ab’)2 anti-rabbit IgG HRP
Predicted band size: 211 kDaResolved by SDS-PAGE on a 4-12% Tris-glycine gel and transferred to PVDF. The membrane was left untreated (Lanes 1-5) or treated with lambda phophatase (Lane 6), blocked with a 5% BSA-TBST buffer for one hour at room temperature and then incubated with ab200809 for two hours at room temperature in 3% BSA-TBST buffer.
N.B. 0.05 μg of PKA (per μg of extract) for 10 minutes at 37 ºC.
