Astana Biomed Group, an authorized Abcam distributor in Central Asia

HRP Anti-non-muscle Myosin IIA antibody [EPR8965] (ab205470)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EPR8965] to non-muscle Myosin IIA
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human
  • Conjugation: HRP

Overview

  • Product name

    HRP Anti-non-muscle Myosin IIA antibody [EPR8965]
    See all non-muscle Myosin IIA primary antibodies

  • Description

    HRP Rabbit monoclonal [EPR8965] to non-muscle Myosin IIA

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat and A431 whole cell and human fetal kidney tissue lysates.

  • General notes

    Alternative versions available:
    Anti-non-muscle Myosin IIA antibody [EPR8965] (ab138498) Knockout validated

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.

  • Storage buffer

    pH: 7.40
    Preservative: 0.1% 10% Proclin 300 Solution
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR8965

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - HRP Anti-non-muscle Myosin IIA antibody [EPR8965] (ab205470)
    Western blot - HRP Anti-non-muscle Myosin IIA antibody [EPR8965] (ab205470)
    All lanes : HRP Anti-non-muscle Myosin IIA antibody [EPR8965] (ab205470) at 1/5000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : MYH9 (non-muscle Myosin IIA) knockout HAP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 227 kDa
    Observed band size: 230 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 seconds


    ab205470 was shown to specifically react with non-muscle Myosin IIA in wild-type HAP1 cells as signal was lost in MYH9 (non-muscle Myosin IIA) knockout cells. Wild-type and MYH9 (non-muscle Myosin IIA) knockout samples were subjected to SDS-PAGE. Ab205470 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.

  • Western blot - HRP Anti-non-muscle Myosin IIA antibody [EPR8965] (ab205470)
    Western blot - HRP Anti-non-muscle Myosin IIA antibody [EPR8965] (ab205470)
    All lanes : HRP Anti-non-muscle Myosin IIA antibody [EPR8965] (ab205470) at 1/5000 dilution

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : Kidney (Human) Tissue Lysate - fetal normal tissue
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 227 kDa
    Observed band size: 230 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab205470 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • HRP Anti-non-muscle Myosin IIA antibody [EPR8965] (ab205470)
    HRP Anti-non-muscle Myosin IIA antibody [EPR8965] (ab205470)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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