HRP Anti-non-muscle Myosin IIA antibody [EPR8965] (ab205470)
![HRP Anti-non-muscle Myosin IIA antibody [EPR8965] (ab205470)](https://www.abcam.com/ps/products/205/ab205470/Images/ab205470-310975-ab205470KOWBMPES.jpg "HRP Anti-non-muscle Myosin IIA antibody [EPR8965] (ab205470)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR8965] to non-muscle Myosin IIA
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-non-muscle Myosin IIA antibody [EPR8965]
See all non-muscle Myosin IIA primary antibodies -
Description
HRP Rabbit monoclonal [EPR8965] to non-muscle Myosin IIA -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Jurkat and A431 whole cell and human fetal kidney tissue lysates.
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General notes
Alternative versions available:
Anti-non-muscle Myosin IIA antibody [EPR8965] (ab138498) Knockout validatedThis product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8965 -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-non-muscle Myosin IIA antibody [EPR8965] (ab205470)All lanes : HRP Anti-non-muscle Myosin IIA antibody [EPR8965] (ab205470) at 1/5000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : MYH9 (non-muscle Myosin IIA) knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 227 kDa
Observed band size: 230 kDa why is the actual band size different from the predicted?
Exposure time: 20 secondsab205470 was shown to specifically react with non-muscle Myosin IIA in wild-type HAP1 cells as signal was lost in MYH9 (non-muscle Myosin IIA) knockout cells. Wild-type and MYH9 (non-muscle Myosin IIA) knockout samples were subjected to SDS-PAGE. Ab205470 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
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![Western blot - HRP Anti-non-muscle Myosin IIA antibody [EPR8965] (ab205470)](https://www.abcam.com/ps/products/205/ab205470/Images/ab205470-257507-WBab2054701.jpg)
Western blot - HRP Anti-non-muscle Myosin IIA antibody [EPR8965] (ab205470)All lanes : HRP Anti-non-muscle Myosin IIA antibody [EPR8965] (ab205470) at 1/5000 dilution
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : Kidney (Human) Tissue Lysate - fetal normal tissue
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 227 kDa
Observed band size: 230 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsThis blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab205470 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-non-muscle Myosin IIA antibody [EPR8965] (ab205470)](https://www.abcam.com/ps/products/205/ab205470/Images/ab205470-4-benefits-of-recombinant-antibodies.png)
HRP Anti-non-muscle Myosin IIA antibody [EPR8965] (ab205470)
