Anti-Fast Myosin Skeletal Heavy chain + MYH4 antibody [EPR22880-64] - BSA and Azide free (ab255685)
![Anti-Fast Myosin Skeletal Heavy chain + MYH4 antibody [EPR22880-64] - BSA and Azide free (ab255685)](https://www.abcam.com/ps/products/255/ab255685/Images/ab255685-2-anti-myh1-myh4-antibody-epr22880-64-immunohistochemistry-skeletal-muscle-human.jpg "Anti-Fast Myosin Skeletal Heavy chain + MYH4 antibody [EPR22880-64] - BSA and Azide free (ab255685)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22880-64] to Fast Myosin Skeletal Heavy chain + MYH4 - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
-
Product name
Anti-Fast Myosin Skeletal Heavy chain + MYH4 antibody [EPR22880-64] - BSA and Azide free -
Description
Rabbit monoclonal [EPR22880-64] to Fast Myosin Skeletal Heavy chain + MYH4 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-Fr or IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Human skeletal muscle tissue lysate. IHC-P: Human skeletal muscle tissue.
-
General notes
Ab255685 is the carrier-free version of ab221149. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab255685 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22880-64 -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fast Myosin Skeletal Heavy chain + MYH4 antibody [EPR22880-64] - BSA and Azide free (ab255685)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Fast Myosin Skeletal Heavy chain + MYH4 with ab221149 at 1/1000 dilution (0.505 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human skeletal muscle (PMID: 30274168, 25217814).
The section was incubated with ab221149 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) .
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221149).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fast Myosin Skeletal Heavy chain + MYH4 antibody [EPR22880-64] - BSA and Azide free (ab255685)](https://www.abcam.com/ps/products/255/ab255685/Images/ab255685-1-anti-myh1-myh4-antibody-epr22880-64-immunohistochemistry-liver-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fast Myosin Skeletal Heavy chain + MYH4 antibody [EPR22880-64] - BSA and Azide free (ab255685)Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Fast Myosin Skeletal Heavy chain + MYH4 with ab221149 at 1/1000 dilution (0.505 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining on human liver (PMID: 30274168). The section was incubated with ab221149 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) .
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221149).
-
![Anti-Fast Myosin Skeletal Heavy chain + MYH4 antibody [EPR22880-64] - BSA and Azide free (ab255685)](https://www.abcam.com/ps/products/255/ab255685/Images/ab255685-3-benefits-of-recombinant-antibodies.png)
Anti-Fast Myosin Skeletal Heavy chain + MYH4 antibody [EPR22880-64] - BSA and Azide free (ab255685)