Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] - BSA and Azide free (ab255278)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22697-17] to Slow Skeletal Myosin Heavy chain - BSA and Azide free
  • Suitable for: IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] - BSA and Azide free
    See all Slow Skeletal Myosin Heavy chain primary antibodies

  • Description

    Rabbit monoclonal [EPR22697-17] to Slow Skeletal Myosin Heavy chain - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IHC-Frmore details
    Unsuitable for: ICC/IF,IP or WB

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human skeletal muscle, cardiac muscle and spleen tissue; Mouse and rat cardiac muscle tissue. IHC-Fr: Mouse and rat skeletal muscle tissue

  • General notes

    ab255278 is the carrier-free version of ab234431.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22697-17

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] - BSA and Azide free (ab255278)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] - BSA and Azide free (ab255278)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Slow Skeletal Myosin Heavy chain using ab234431 at 1/2000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on the human skeletal muscle (PMID: 22530000). The section was incubated with ab234431 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234431).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] - BSA and Azide free (ab255278)
    Immunohistochemistry (Frozen sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] - BSA and Azide free (ab255278)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed 0.2% Triton X-100 permeabilized rat skeletal muscle tissue labeling Slow Skeletal Myosin Heavy chain using ab234431 at 1/2000 dilution (green), followed by an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.  Cytoplasmic staining on the rat skeletal muscle (PMID: 29193153). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234431).

  • Immunohistochemistry (Frozen sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] - BSA and Azide free (ab255278)
    Immunohistochemistry (Frozen sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] - BSA and Azide free (ab255278)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed 0.2% Triton X-100 permeabilized mouse skeletal muscle tissue labeling Slow Skeletal Myosin Heavy chain using ab234431 at 1/2000 dilution (green), followed by an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.  Cytoplasmic staining on the mouse skeletal muscle (PMID: 29193153). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234431).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] - BSA and Azide free (ab255278)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] - BSA and Azide free (ab255278)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Slow Skeletal Myosin Heavy chain using ab234431 at 1/2000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative staining on the smooth muscle in the human spleen (PMID: 22530000). The section was incubated with ab234431 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234431).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] - BSA and Azide free (ab255278)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] - BSA and Azide free (ab255278)

    Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling Slow Skeletal Myosin Heavy chain using ab234431 at 1/2000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on the rat cardiac muscle (PMID: 22530000). The section was incubated with ab234431 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234431).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] - BSA and Azide free (ab255278)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] - BSA and Azide free (ab255278)

    Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Slow Skeletal Myosin Heavy chain using ab234431 at 1/2000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on the mouse cardiac muscle (PMID: 22530000). The section was incubated with ab234431 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234431).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] - BSA and Azide free (ab255278)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] - BSA and Azide free (ab255278)

    Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling Slow Skeletal Myosin Heavy chain using ab234431 at 1/2000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on the human cardiac muscle (PMID: 22530000). The section was incubated with ab234431 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234431).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] - BSA and Azide free (ab255278)
    Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] - BSA and Azide free (ab255278)

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