Anti-Myosin light chain (phospho S1) antibody (ab157747)
Key features and details
- Rabbit polyclonal to Myosin light chain (phospho S1)
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-Myosin light chain (phospho S1) antibody
See all Myosin light chain primary antibodies -
Description
Rabbit polyclonal to Myosin light chain (phospho S1) -
Host species
Rabbit -
Specificity
The immunogen sequence is highly conserved in Human, rat and mouse MYL9, and in other smooth muscle and non-muscle Myosin light chains. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF RatWB Mouse
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Immunogen
Synthetic peptide corresponding to Cow Myosin light chain (N terminal) conjugated to keyhole limpet haemocyanin.
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Positive control
- A7r5 cells; EGF or calyculin A treated Human A431 cell lysates; Mouse C2C12, rat PC12, and rabbit fibroblasts treated with calyculin A.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 0.1% BSA, 49% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab157747 was affinity purified using phospho-Myosin light chain (Ser-1) peptide (without carrier). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Myosin light chain (phospho S1) antibody (ab157747)Immunofluorescent analysis of paraformaldehyde-fixed A7r5 cells labeling Myosin light chain with ab157747 at 1/100 dilution (left). Cells were dual stained with mouse anti-Myosin light chain antibody (middle panel) and the overlay of staining patterns is shown to the right. Goat anti-mouse Dylight 488 and goat anti-rabbit Dylight 594 were used for detection of primary antibodies.
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Western blot - Anti-Myosin light chain (phospho S1) antibody (ab157747)All lanes : Anti-Myosin light chain (phospho S1) antibody (ab157747)
Lane 1 : C2C12 cell lysate, untreated
Lane 2 : C2C12 cell lysate treated
with Lambda phosphatase
Predicted band size: 20 kDa
