Human Myosin light chain 3 ELISA Kit (ab246538)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 89 pg/ml
- Range: 125 pg/ml - 8000 pg/ml
- Sample type: Cell culture extracts, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human Myosin light chain 3 ELISA Kit
See all Myosin light chain 3 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% extract 8 1.9% Inter-assay Sample n Mean SD CV% extract 3 1% -
Sample type
Cell culture extracts, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
89 pg/ml -
Range
125 pg/ml - 8000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Tissue Extracts 95 92% - 100% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Myosin light chain 3 ELISA Kit (ab246538) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Myosin light chain 3 protein in tissue extracts and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Myosin light chain 3 with 89 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Myosin light chain 3 Capture Antibody 1 x 600µl 10X Human Myosin light chain 3 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human Myosin light chain 3 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
-
Function
Regulatory light chain of myosin. Does not bind calcium. -
Involvement in disease
Defects in MYL3 are the cause of cardiomyopathy familial hypertrophic type 8 (CMH8) [MIM:608751]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. CMH8 inheritance can be autosomal dominant or recessive.
Defects in MYL3 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 1 (MVC1) [MIM:608751]. MVC1 is a very rare variant of familial hypertrophic cardiomyopathy, characterized by mid-left ventricular chamber thickening. -
Sequence similarities
Contains 3 EF-hand domains. -
Post-translational
modificationsThe N-terminus is blocked.
N-terminus is methylated by METTL11A/NTM1. - Information by UniProt
-
Alternative names
- Cardiac myosin light chain 1
- CMH8
- CMLC1
see all -
Database links
- Entrez Gene: 4634 Human
- Omim: 160790 Human
- SwissProt: P08590 Human
- Unigene: 517939 Human
Images
-
Other - Human Myosin light chain 3 ELISA Kit (ab246538)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Example of human Myosin Light Chain 3 standard curve in 1X Cell Extraction Buffer PTR.The Myosin Light Chain 3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
-
Interpolated concentrations of native Myosin Light Chain 3 in human heart and skeletal muscle extract samples based on a 72 µg/mL and 20 µg/mL extract load respectively.The concentrations of Myosin Light Chain 3 were measured in duplicate and interpolated from the Myosin Light Chain 3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Myosin Light Chain 3 concentration was determined to be 1538 pg/mL in heart extract and 2893 pg/mL in skeletal muscle extract.
-
Sandwich ELISA - Human Myosin light chain 3 ELISA Kit (ab246538)To learn more about the advantages of recombinant antibodies see here.
