Anti-Myosin Light Chain 2 antibody (ab48003)
Key features and details
- Rabbit polyclonal to Myosin Light Chain 2
- Suitable for: ICC/IF, WB, ELISA, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Myosin Light Chain 2 antibody
See all Myosin Light Chain 2 primary antibodies -
Description
Rabbit polyclonal to Myosin Light Chain 2 -
Host species
Rabbit -
Specificity
ab48003 is directed against the regulatory light chain of smooth and non muscle myosin. The antibody detects both unphosphorylated and monophosphorylated forms of the protein. -
Tested applications
Suitable for: ICC/IF, WB, ELISA, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Dog, Pig, Xenopus laevis, Zebrafish
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Immunogen
Synthetic peptide:
GANSNVFSMFEQTQIQ
, corresponding to amino acids 12/27 of Human Myosin Light Chain 2 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Myosin Light Chain 2 antibody (ab48003)All lanes : Anti-Myosin Light Chain 2 antibody (ab48003) at 1/600 dilution
Lane 1 : Vascular myosin (rat aorta), loaded with 35 µg of lysate
Lane 2 : Cardiac myosin (mouse heart, loaded with 35 µg of lysate
Secondary
All lanes : 1:10,000 dilution of
IRDye800™ conjugated Gt-a-Rabbit IgG [H&L]
Predicted band size: 19 kDa
Observed band size: 19,24 kDa why is the actual band size different from the predicted?
Arrowheads indicate the detection of both monophosphorylated (upper) and unphosphorylated (lower) forms of the protein. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody (ab48003)ab48003 (2µg/ml) staining Myosin Light Chain 2 in human skeletal muscle using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of cell cytoplasm.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
