Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)
![Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)](https://www.abcam.com/ps/products/92/ab92721/Images/ab92721-335199-anti-myosin-light-chain-2-antibody-epr3741-western-blot.jpg "Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3741] to Myosin Light Chain 2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Myosin Light Chain 2 antibody [EPR3741]
See all Myosin Light Chain 2 primary antibodies -
Description
Rabbit monoclonal [EPR3741] to Myosin Light Chain 2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB MouseRatHuman
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Immunogen
Synthetic peptide within Human Myosin Light Chain 2 aa 150-250. The exact sequence is proprietary.
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Positive control
- WB: Recombinant Human Myosin Light Chain 2 protein (ab117178), Fetal heart, fetal skeletal muscle, Human heart, mouse heart and rat heart lysates IHC-P: Human heart muscle
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.78 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3741 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)All lanes : Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721) at 1/1000 dilution
Lane 1 : Human fetal heart tissue lysate
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : 293T (Human embryonic kidney epithelial cell), whole cell lysate
Lane 4 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 19 kDa
Observed band size: 19 kDa
Exposure time: 10 secondsBlocking/Diluting buffer and concentration 5% NFDM/TBST
Myosin light chain-2 (MYL2, also called MLC-2) is an ~19kDa sarcomeric protein that belongs to the EF-hand calcium binding protein superfamily and exists as three major isoforms encoded by three distinct genes in mammalian striated muscle (PMID: 26074085). Although some cell lines are reported to be MYL2 positive, such as HeLa, 293T and MCF7 (PMID: 21487036, 21487036, 23093227), ab92721 only works in muscle and heart tissues according to our internal testing.
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![Western blot - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)](https://www.abcam.com/ps/products/92/ab92721/Images/ab92721-107922-anti-myosin-light-chain-2-antibody-epr3741-western-blot.jpg)
Western blot - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)All lanes : Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721) at 1/10000 dilution
Lane 1 : Fetal heart lysate
Lane 2 : Fetal skeletal muscle lysate
Lane 3 : Human heart lysate
Lane 4 : Mouse heart lysate
Lane 5 : Rat heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 19 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)](https://www.abcam.com/ps/products/92/ab92721/Images/ab92721-107921-anti-myosin-light-chain-2-antibody-epr3741-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)ab92721, at a 1/100 dilution, staining Myosin Light Chain 2 in paraffin embedded Human heart muscle by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)](https://www.abcam.com/ps/products/92/ab92721/Images/ab92721-107920-anti-myosin-light-chain-2-antibody-epr3741-western-blot.jpg)
Western blot - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)All lanes : Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721) at 1/10000 dilution
Lane 1 :Recombinant Human Myosin Light Chain 2 protein (ab79185) at 0.1 µg
Lane 2 :Recombinant Human Myosin Light Chain 2 protein (ab79185) at 0.01 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 19 kDa
Exposure time: 4 minutes -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)](https://www.abcam.com/ps/products/92/ab92721/Images/ab92721-107919-anti-myosin-light-chain-2-antibody-epr3741-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)ab92721 showing positive staining in human Heart muscle tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)](https://www.abcam.com/ps/products/92/ab92721/Images/ab92721-107918-anti-myosin-light-chain-2-antibody-epr3741-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)ab92721 showing positive staining in human Skeletal muscle tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)](https://www.abcam.com/ps/products/92/ab92721/Images/ab92721-107917-anti-myosin-light-chain-2-antibody-epr3741-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)ab92721 showing negative staining in Normal human tonsil tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)](https://www.abcam.com/ps/products/92/ab92721/Images/ab92721-107916-anti-myosin-light-chain-2-antibody-epr3741-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)ab92721 showing negative staining in Normal human brain tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)](https://www.abcam.com/ps/products/92/ab92721/Images/ab92721-107915-anti-myosin-light-chain-2-antibody-epr3741-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)ab92721 showing negative staining in Normal human liver tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)](https://www.abcam.com/ps/products/92/ab92721/Images/ab92721-107914-anti-myosin-light-chain-2-antibody-epr3741-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)ab92721 showing negative staining in Normal human kidney tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)](https://www.abcam.com/ps/products/92/ab92721/Images/ab92721-107913-anti-myosin-light-chain-2-antibody-epr3741-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)ab92721 showing negative staining in Normal human breast tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)](https://www.abcam.com/ps/products/92/ab92721/Images/ab92721-107912-anti-myosin-light-chain-2-antibody-epr3741.jpg)
OI-RD Scanning - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721) -
![Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)](https://www.abcam.com/ps/products/92/ab92721/Images/ab92721-14-benefits-of-recombinant-antibodies.png)
Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)
