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Anti-non-muscle Myosin IIA antibody [EPR22933-9] - BSA and Azide free (ab256477)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22933-9] to non-muscle Myosin IIA - BSA and Azide free
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-non-muscle Myosin IIA antibody [EPR22933-9] - BSA and Azide free
    See all non-muscle Myosin IIA primary antibodies

  • Description

    Rabbit monoclonal [EPR22933-9] to non-muscle Myosin IIA - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    IHC is not recommended for human.

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human liver and lung tissue lysates; mouse lung tissue lysate; rat spleen and lung tissue lysates; Neuro-2a, HEK-292T and HeLa whole cell lysates. IHC-P: Mouse liver tissue; rat liver tissue.

  • General notes

    ab256477 is the carrier-free version of ab238131.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22933-9

  • Isotype

    IgG

  • Research areas

Images

  • Immunoprecipitation - Anti-non-muscle Myosin IIA antibody [EPR22933-9] - BSA and Azide free (ab256477)
    Immunoprecipitation - Anti-non-muscle Myosin IIA antibody [EPR22933-9] - BSA and Azide free (ab256477)

    non-muscle Myosin IIA was immunoprecipitated from 0.35 mg NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate 10ug with ab238131 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab238131. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate 10ug

    Lane 2: ab238131 IP in NIH/3T3 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab238131 in NIH/3T3 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238131).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR22933-9] - BSA and Azide free (ab256477)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR22933-9] - BSA and Azide free (ab256477)

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling non-muscle Myosin IIA with ab238131 at 1/500 dilution (0.854 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat liver (PMID: 11943476, 22558294, 17668046). The section was incubated with ab238131 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238131).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR22933-9] - BSA and Azide free (ab256477)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR22933-9] - BSA and Azide free (ab256477)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling non-muscle Myosin IIA with ab238131 at 1/500 dilution (0.854 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse liver (PMID: 11943476, 22558294, 17668046). The section was incubated with ab238131 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control/ Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238131).

  • Anti-non-muscle Myosin IIA antibody [EPR22933-9] - BSA and Azide free (ab256477)
    Anti-non-muscle Myosin IIA antibody [EPR22933-9] - BSA and Azide free (ab256477)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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