Human Myosin light chain 3 Antibody Pair - BSA and Azide free (ab256683)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Human
Overview
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Product name
Human Myosin light chain 3 Antibody Pair - BSA and Azide free
See all Myosin light chain 3 kits -
Assay type
ELISA set -
Range
125 pg/ml - 8000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
The Antibody Pair can be used to quantify Human Myosin light chain 3. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab246538), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Human Myosin light chain 3 Capture Antibody (unconjugated) 1 x 100µg Human Myosin light chain 3 Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Function
Regulatory light chain of myosin. Does not bind calcium. -
Involvement in disease
Defects in MYL3 are the cause of cardiomyopathy familial hypertrophic type 8 (CMH8) [MIM:608751]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. CMH8 inheritance can be autosomal dominant or recessive.
Defects in MYL3 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 1 (MVC1) [MIM:608751]. MVC1 is a very rare variant of familial hypertrophic cardiomyopathy, characterized by mid-left ventricular chamber thickening. -
Sequence similarities
Contains 3 EF-hand domains. -
Post-translational
modificationsThe N-terminus is blocked.
N-terminus is methylated by METTL11A/NTM1. - Information by UniProt
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Alternative names
- Cardiac myosin light chain 1
- CMH8
- CMLC1
see all -
Database links
- Entrez Gene: 4634 Human
- Omim: 160790 Human
- SwissProt: P08590 Human
- Unigene: 517939 Human
Images
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Sandwich ELISA - Human Myosin light chain 3 Antibody Pair - BSA and Azide free (ab256683)To learn more about the advantages of recombinant antibodies see here.
