Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MYH2 antibody [A4.74] - BSA and Azide free (ab264037)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [A4.74] to MYH2 - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-MYH2 antibody [A4.74] - BSA and Azide free
    See all MYH2 primary antibodies

  • Description

    Mouse monoclonal [A4.74] to MYH2 - BSA and Azide free

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Full length native protein (purified) within Human MYH2. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q9UKX2

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: Human skeletal muscle tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab264037 is the carrier-free version of ab264036. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH2 antibody [A4.74] - BSA and Azide free (ab264037)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH2 antibody [A4.74] - BSA and Azide free (ab264037)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Myosin (MYH2) with ab264036 at 1/1000 dilution (1.116µg/ml) followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on human skeletal muscle. The section was incubated with ab264036 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab264036).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH2 antibody [A4.74] - BSA and Azide free (ab264037)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH2 antibody [A4.74] - BSA and Azide free (ab264037)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Myosin (MYH2) with ab264036 at 1/1000 dilution (1.116µg/ml) followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). The section was incubated with ab264036 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Negative control: No staining on human liver (PMID:12899730).  

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab264036).

  • Anti-MYH2 antibody [A4.74] - BSA and Azide free (ab264037)
    Anti-MYH2 antibody [A4.74] - BSA and Azide free (ab264037)

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