Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BMP9 antibody [EPR20009] - BSA and Azide free (ab251471)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20009] to BMP9 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-BMP9 antibody [EPR20009] - BSA and Azide free
    See all BMP9 primary antibodies

  • Description

    Rabbit monoclonal [EPR20009] to BMP9 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251471 is the carrier-free version of ab207318. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251471 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-BMP9 antibody [EPR20009] - BSA and Azide free (ab251471)
    Western blot - Anti-BMP9 antibody [EPR20009] - BSA and Azide free (ab251471)
    All lanes : Anti-BMP9 antibody [EPR20009] (ab207318) at 1/10000 dilution

    Lane 1 : GST-tagged Human BMP9 recombinant protein (aa338-429)
    Lane 2 : His-tagged Human BMP10 recombinant protein (aa334-424)
    Lane 3 : His-tagged Human BMP13 recombinant protein (aa365-455)

    Lysates/proteins at 0.02 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 47 kDa
    Observed band size: 35 kDa
    why is the actual band size different from the predicted?


    Exposure time: 4 seconds


    This data was developed using ab207318, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-BMP9 antibody [EPR20009] - BSA and Azide free (ab251471)
    Western blot - Anti-BMP9 antibody [EPR20009] - BSA and Azide free (ab251471)
    All lanes : Anti-BMP9 antibody [EPR20009] (ab207318) at 1/1000 dilution

    Lane 1 : Human liver lysate
    Lane 2 : Human brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 47 kDa
    Observed band size: 47 kDa


    Exposure time: 3 minutes


    This data was developed using ab207318, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    BMP9 is mostly expressed in liver and has very low expression levels in brain and other tissues (PMID 21710321; 23812757).

  • Anti-BMP9 antibody [EPR20009] - BSA and Azide free (ab251471)
    Anti-BMP9 antibody [EPR20009] - BSA and Azide free (ab251471)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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