Anti-BMP6 antibody [morph-6.1] (ab15640)
![Anti-BMP6 antibody [morph-6.1] (ab15640)](https://www.abcam.com/ps/products/15/ab15640/Images/ab15640-23297-anti-bmp6-antibody-morph-61-immunofluorescence.jpg "Anti-BMP6 antibody [morph-6.1] (ab15640)")
Key features and details
- Mouse monoclonal [morph-6.1] to BMP6
- Suitable for: ICC/IF
- Reacts with: Rat
- Isotype: IgG1
Overview
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Product name
Anti-BMP6 antibody [morph-6.1]
See all BMP6 primary antibodies -
Description
Mouse monoclonal [morph-6.1] to BMP6 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Synthetic peptide:
MPGLGRRAQWLCWWWGLLCSCCGPPPLRP
conjugated to KLH, corresponding to amino acids 1-29 of Rat BMP6. -
Positive control
- Positive staining can be observed in embryonic and adult central nervous system. Rat radial glial cells of the developing central nervous system from E11 to E19. In rat peripheral nerves a selective intracellular immunoreactivity can be found in perinuclear region of most Schwann cells which form the myelin sheath. However, some Schwann cells were negative for the antigen.
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General notes
Pre-incubation of anti BMP6 with amino-terminal peptide 1-29 of BMP6 inhibits binding of the mAb to tissue sections. Pre-incubation with other amino-terminal synthetic peptides of human BMP7, BMP4, BMP3 and BMP2 as well as an irrelevant peptide does not inhibit specific tissue staining.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.05% KATHON™ CG/ICP
Constituents: 0.5% BSA, 99% PBS -
Concentration information loading... -
Purity
Affinity purified -
Primary antibody notes
Pre-incubation of anti BMP6 with amino-terminal peptide 1-29 of BMP6 inhibits binding of the mAb to tissue sections. Pre-incubation with other amino-terminal synthetic peptides of human BMP7, BMP4, BMP3 and BMP2 as well as an irrelevant peptide does not inhibit specific tissue staining. -
Clonality
Monoclonal -
Clone number
morph-6.1 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-BMP6 antibody [morph-6.1] (ab15640) This image is courtesy of an anonymous Abreview
ab15640 staining human U2-OS cells by ICC/IF. Cells were PFA fixed and permeabilized in Triton-X-100 prior to blocking in 2% serum for 1 hour at 20°C. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 37°C. The secondary antibody was Alexa Fluor®488 conjugated chicken anti-mouse, diluted 1/1000.
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![Immunocytochemistry/ Immunofluorescence - Anti-BMP6 antibody [morph-6.1] (ab15640)](https://www.abcam.com/ps/products/15/ab15640/Images/ab15640-23295-anti-bmp6-antibody-morph-61-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-BMP6 antibody [morph-6.1] (ab15640) This image is courtesy of Ruma Raha-Chowdhury, University Of Cambridge, United KingdomICC/IF image of ab15640 staining primary rat myoblast cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 5% normal donkey serum / 0.3M glycine in 0.1% PBS-Tritonx100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15640, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488donkey anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h.
