Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6756(B)] to Glypican 5
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Glypican 5 antibody [EPR6756(B)]
    See all Glypican 5 primary antibodies

  • Description

    Rabbit monoclonal [EPR6756(B)] to Glypican 5

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Glypican 5 aa 1-100 (internal sequence). The exact sequence is proprietary.

  • Positive control

    • A549, Human fetal lung, HepG2 and C6 lysates; Human brain tissue

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 6.98 x 10 -11 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA, 49% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR6756(B)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)
    Western blot - Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)
    All lanes : Anti-Glypican 5 antibody [EPR6756(B)] (ab124886) at 1/1000 dilution (Unpurified)

    Lane 1 : A549 cell lysate
    Lane 2 : Human fetal lung lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : C6 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 64 kDa
    Observed band size: 63 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)

    Unpurified ab124886, at 1/250 dilution, staining Glypican 5 in paraffin-embedded Human brain tissue by Immunohistochemistry.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)
    Immunohistochemical staining of paraffin embedded human glioma with purified ab124886 at a working dilution of 1/100. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Western blot - Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)
    Western blot - Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)
    All lanes : Anti-Glypican 5 antibody [EPR6756(B)] (ab124886) at 1/1000 dilution (purified)

    Lane 1 : C6 whole cell lysate
    Lane 2 : Mouse spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 64 kDa
    Observed band size: 64 kDa



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)
    Western blot - Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)
    Anti-Glypican 5 antibody [EPR6756(B)] (ab124886) at 1/1000 dilution (purified) + Human fetal lung at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 64 kDa
    Observed band size: 64 kDa



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)
    Western blot - Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)
    Anti-Glypican 5 antibody [EPR6756(B)] (ab124886) at 1/1000 dilution (purified) + A549 whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 64 kDa
    Observed band size: 64 kDa



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • OI-RD Scanning - Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)
    OI-RD Scanning - Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)
    Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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