Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Sulfatase 1/SULF1 antibody (ab32763)

Key features and details

  • Rabbit polyclonal to Sulfatase 1/SULF1
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Sulfatase 1/SULF1 antibody
    See all Sulfatase 1/SULF1 primary antibodies

  • Description

    Rabbit polyclonal to Sulfatase 1/SULF1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, Quail

  • Immunogen

    Synthetic peptide corresponding to Human Sulfatase 1/SULF1 aa 850 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab32762)

  • Positive control

    • IHC-P: Normal human skeletal muscle.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab32763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (2)
Use a concentration of 0.1 - 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
IHC-P
Use a concentration of 0.1 - 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin. Diminishes HSPG (heparan sulfate proteoglycans) sulfation, inhibits signaling by heparin-dependent growth factors, diminishes proliferation, and facilitates apoptosis in response to exogenous stimulation.

  • Tissue specificity

    Expressed at highest levels in testis, stomach, skeletal muscle, lung, kidney, pancreas, small intestine and colon. It is also detected in normal ovarian surface epithelial cells. Down-regulation seen in ovarian carcinoma cell lines, ovarian cancers, breast, pancreatic, renal and hepatocellular carcinoma cell lines.

  • Sequence similarities

    Belongs to the sulfatase family.

  • Post-translational
    modifications

    The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.

  • Cellular localization

    Endoplasmic reticulum. Golgi apparatus > Golgi stack. Cell surface. Also localized on the cell surface.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • DSulf antibody
    • Extracellular sulfatase Sulf 1 antibody
    • Extracellular sulfatase Sulf-1 antibody
    • Extracellular sulfatase Sulf1 antibody
    • HSULF 1 antibody
    • HSulf-1 antibody
    • Neu5 antibody
    • Sulf1 antibody
    • SULF1_HUMAN antibody
    • Sulfatase FP antibody
    • Sulfated antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sulfatase 1/SULF1 antibody (ab32763)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sulfatase 1/SULF1 antibody (ab32763)

    IHC image of Sulfatase 1/SULF1 staining in Human skeletal muscle formalin-fixed paraffin embedded tissue section*, performed on a Leica Bond™ system.. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32763, 0.1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. 

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

References (9)

Publishing research using ab32763? Please let us know so that we can cite the reference in this datasheet.

ab32763 has been referenced in 9 publications.

  • Hu Z  et al. Single-Cell Transcriptomic Atlas of Different Human Cardiac Arteries Identifies Cell Types Associated With Vascular Physiology. Arterioscler Thromb Vasc Biol 41:1408-1427 (2021). PubMed: 33626908
  • Chen F  et al. HSulf-1 and palbociclib exert synergistic antitumor effects on RB-positive triple-negative breast cancer. Int J Oncol 57:223-236 (2020). PubMed: 32377705
  • Perkins TN  et al. Crystalline silica alters Sulfatase-1 expression in rat lungs which influences hyper-proliferative and fibrogenic effects in human lung epithelial cells. Toxicol Appl Pharmacol 348:43-53 (2018). PubMed: 29673857
  • Yamada T  et al. Heparan sulfate alterations in extracellular matrix structures and fibroblast growth factor-2 signaling impairment in the aged neurogenic niche. J Neurochem 142:534-544 (2017). PubMed: 28547849
  • Okada T  et al. Desulfation of Heparan Sulfate by Sulf1 and Sulf2 Is Required for Corticospinal Tract Formation. Sci Rep 7:13847 (2017). PubMed: 29062064
  • Cheriyan VT  et al. Disulfiram suppresses growth of the malignant pleural mesothelioma cells in part by inducing apoptosis. PLoS One 9:e93711 (2014). WB ; Human . PubMed: 24690739
  • Ji W  et al. hSulf-1 gene exhibits anticancer efficacy through negatively regulating VEGFR-2 signaling in human cancers. PLoS One 6:e23274 (2011). PubMed: 21853101
  • Ratelade J  et al. A murine model of Denys-Drash syndrome reveals novel transcriptional targets of WT1 in podocytes. Hum Mol Genet 19:1-15 (2010). IHC-Fr ; Mouse . PubMed: 19797313
  • Otsuki S  et al. Extracellular sulfatases support cartilage homeostasis by regulating BMP and FGF signaling pathways. Proc Natl Acad Sci U S A 107:10202-7 (2010). WB ; Human . PubMed: 20479257

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sulfatase 1/SULF1 antibody (ab32763)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sulfatase 1/SULF1 antibody (ab32763)

    IHC image of Sulfatase 1/SULF1 staining in Human skeletal muscle formalin-fixed paraffin embedded tissue section*, performed on a Leica Bond™ system.. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32763, 0.1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. 

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

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